The aim of this work was the fabrication and characterization of bioactive glass–poly(lactic-co-glycolic acid) (PLGA) composite scaffolds mimicking the topological features of cancellous bone. Porous multilayer PLGA–CEL2 composite scaffolds were innovatively produced by a pressure-activated microsyringe (PAM) method, a CAD/CAM processing technique originally developed at the University of Pisa. In order to select the optimal formulations to be extruded by PAM, CEL2–PLGA composite films (CEL2 is an experimental bioactive SiO2–P2O5–CaO–MgO–Na2O–K2O glass developed at Politecnico di Torino) were produced and mechanically tested. The elastic modulus of the films increased from 30 to>400MPa, increasing the CEL2 amount (10–50wt%) in the composite. The mixture containing 20 wt% CEL2 was used to fabricate 2D and 3D bone-like scaffolds composed by layers with different topologies (square, hexagonal and octagonal pores). It was observed that the increase of complexity of 2D topological structures led to an increment of the elastic modulus from 3 to 9 MPa in the com- posite porous monolayer. The elastic modulus of 3D multilayer scaffolds was intermediate (about 6.5 MPa) between the values of the monolayers with square and octagonal pores (corresponding to the lowest and highest complexity, respectively). MG63 osteoblast-like cells and periosteal-derived precursor cells (PDPCs) were used to assess the biocompatibility of the 3D bone-like scaffolds. A significant increase in cell proliferation between 48 h and 7 days of culture was observed for both cell phenotypes. Moreover, qRT–PCR analysis evidenced an induction of early genes of osteogenesis in PDPCs.

Pressure-activated microsyringe (PAM) fabrication of bioactive glass-poly(lactic-co-glycolic acid) composite scaffolds for bone tissue regeneration

DE MARIA, CARMELO;VOZZI, GIOVANNI
2017-01-01

Abstract

The aim of this work was the fabrication and characterization of bioactive glass–poly(lactic-co-glycolic acid) (PLGA) composite scaffolds mimicking the topological features of cancellous bone. Porous multilayer PLGA–CEL2 composite scaffolds were innovatively produced by a pressure-activated microsyringe (PAM) method, a CAD/CAM processing technique originally developed at the University of Pisa. In order to select the optimal formulations to be extruded by PAM, CEL2–PLGA composite films (CEL2 is an experimental bioactive SiO2–P2O5–CaO–MgO–Na2O–K2O glass developed at Politecnico di Torino) were produced and mechanically tested. The elastic modulus of the films increased from 30 to>400MPa, increasing the CEL2 amount (10–50wt%) in the composite. The mixture containing 20 wt% CEL2 was used to fabricate 2D and 3D bone-like scaffolds composed by layers with different topologies (square, hexagonal and octagonal pores). It was observed that the increase of complexity of 2D topological structures led to an increment of the elastic modulus from 3 to 9 MPa in the com- posite porous monolayer. The elastic modulus of 3D multilayer scaffolds was intermediate (about 6.5 MPa) between the values of the monolayers with square and octagonal pores (corresponding to the lowest and highest complexity, respectively). MG63 osteoblast-like cells and periosteal-derived precursor cells (PDPCs) were used to assess the biocompatibility of the 3D bone-like scaffolds. A significant increase in cell proliferation between 48 h and 7 days of culture was observed for both cell phenotypes. Moreover, qRT–PCR analysis evidenced an induction of early genes of osteogenesis in PDPCs.
2017
Mattioli Belmonte, M.; DE MARIA, Carmelo; Vitale Brovarone, C.; Baino, F.; Dicarlo, M.; Vozzi, Giovanni
File in questo prodotto:
File Dimensione Formato  
Pressure-activated microsyringe (PAM) fabrication of bioactive glasspoly(lactic-co-glycolic acid) composite scaffolds for bone tissue regeneration.pdf

accesso aperto

Descrizione: preprint del paper
Tipologia: Documento in Pre-print
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.59 MB
Formato Adobe PDF
1.59 MB Adobe PDF Visualizza/Apri
term.2095.pdf

solo utenti autorizzati

Descrizione: published paper
Tipologia: Versione finale editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 559.94 kB
Formato Adobe PDF
559.94 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/765294
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 18
  • ???jsp.display-item.citation.isi??? 17
social impact