Morpho-anatomical and physiological traits of Agrostis castellana living in an active geothermal alteration field

Agrostis castellana is one of the few plants colonizing one of the most extreme geothermal alteration fields characterized by low pH and high temperature of soil. The study of species surviving in these multi-stress habitats can help to know more in deep the adaptive ability of plants. In this work morpho-anatomical, functional and physiological traits of leaves of A. castellana living few meters from fumaroles were studied, focusing on their putative ecological significance. Some typical xeromorphic traits occurred in the leaves of these plants: abundant cutinization, cell wall thickening, slightly convolute and adaxially ribbed leaf blades, tissutal reinforcements by sclereids, pubescence, protected stomata and densely packed mesophyll. Abundant pectins, important in both water balance adjusting and as monosaccharide source, were observed in epidermal cell walls. Despite the low value of relative water content, indicative of a disturbed hydric state, oxidative damage, in terms of lipid peroxidation, indicative of membrane damage, was significantly lower than in other plants of the same environment, probably tanks to an adequate antioxidant response based mainly on enzymatic machinery. Interestingly catalase activity was not inhibited by extreme conditions of the geothermal alteration field as in other plants of the same habitat. In conclusion, a cooperation of xeromorphic traits and antioxidant response seems to make A. castellana able to survive in such a prohibitive environment.