The purification of the thyroid TSH receptor has been described using bovine thyroid plasma mem- branes and bovine TSH [1]. Studies on its characteri- zation required a large amount of thyroid tissue because of the low yield of purified material in terms of protein concentration. The use of radiolabelled material for purification may be advantageous, since very small amounts of labelled protein can be easily detected. Radioiodination of cell surface components or plasma membrane purified materials by enzymic procedures has been widely used for the study of sur- face proteins of human erythrocytes [2] and human platelet membrane [3,4]. In the present report human thyroid plasma mem- branes were radioiodinated, solubilized and then used for the purification of the TSH receptor by affinity chromatography with TSH-Sepharose column. The radiolabeUed plasma membrane material was also studied by conventional chromatography on agarose gel
Use of solubilized radioiodinated thyroid plasma membranes for purification of TSH-receptor by affinity chromatography
MACCHIA, ENRICO;BARTALENA, LUIGI;MONZANI, FABIO;PINCHERA, ALDO
1978-01-01
Abstract
The purification of the thyroid TSH receptor has been described using bovine thyroid plasma mem- branes and bovine TSH [1]. Studies on its characteri- zation required a large amount of thyroid tissue because of the low yield of purified material in terms of protein concentration. The use of radiolabelled material for purification may be advantageous, since very small amounts of labelled protein can be easily detected. Radioiodination of cell surface components or plasma membrane purified materials by enzymic procedures has been widely used for the study of sur- face proteins of human erythrocytes [2] and human platelet membrane [3,4]. In the present report human thyroid plasma mem- branes were radioiodinated, solubilized and then used for the purification of the TSH receptor by affinity chromatography with TSH-Sepharose column. The radiolabeUed plasma membrane material was also studied by conventional chromatography on agarose gelI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
