Titanium dioxide nanoparticles (TiO2-NPs) continuously released into waters, may cause harmful effects to marine organisms and their potential interaction with conventional toxic contaminants represents a growing concern for biota. We investigated the genotoxic potential of nanosized titanium dioxide (n-TiO2) (100 μg L(-1)) alone and in combination with CdCl2 (100 μg L(-1)) in Mytilus galloprovincialis after 4 days of in vivo exposure. RAPD-PCR technique and Micronucleus test were used to study genotoxicity. The results showed genome template stability (GTS) being markedly reduced after single exposure to n-TiO2 and CdCl2. Otherwise, co-exposure resulted in a milder reduction of GTS. Exposure to n-TiO2 was responsible for a significant increase of micronucleated cell frequency in gill tissue, while no chromosomal damage was observed after CdCl2 exposure as well as after combined exposure to both substances. Copyright © 2015 Elsevier Ltd. All rights reserved.

Genomic and chromosomal damage in the marine mussel Mytilus galloprovincialis: Effects of the combined exposure to titanium dioxide nanoparticles and cadmium chloride.

NIGRO, MARCO;BERNARDESCHI, MARGHERITA;GUIDI, PATRIZIA;SCARCELLI, VITTORIA;FRENZILLI, GIADA
2015-01-01

Abstract

Titanium dioxide nanoparticles (TiO2-NPs) continuously released into waters, may cause harmful effects to marine organisms and their potential interaction with conventional toxic contaminants represents a growing concern for biota. We investigated the genotoxic potential of nanosized titanium dioxide (n-TiO2) (100 μg L(-1)) alone and in combination with CdCl2 (100 μg L(-1)) in Mytilus galloprovincialis after 4 days of in vivo exposure. RAPD-PCR technique and Micronucleus test were used to study genotoxicity. The results showed genome template stability (GTS) being markedly reduced after single exposure to n-TiO2 and CdCl2. Otherwise, co-exposure resulted in a milder reduction of GTS. Exposure to n-TiO2 was responsible for a significant increase of micronucleated cell frequency in gill tissue, while no chromosomal damage was observed after CdCl2 exposure as well as after combined exposure to both substances. Copyright © 2015 Elsevier Ltd. All rights reserved.
2015
Rocco, L; Santonastaso, M; Nigro, Marco; Mottola, F; Costagliola, D; Bernardeschi, Margherita; Guidi, Patrizia; Lucchesi, Paolo; Scarcelli, Vittoria; Corsi, I; Stingo, V; Frenzilli, Giada
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S014111361530043X-main.pdf

solo utenti autorizzati

Descrizione: articolo principale
Tipologia: Versione finale editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 485.3 kB
Formato Adobe PDF
485.3 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/782517
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 31
social impact