Removal of seminal plasma (SP) before cryopreservation or its addition after thawing affected pregnancy rates and uterine reaction after frozen semen artificial insemination [1-4]. Aims of this study were to evaluate the differences in pregnancy rates and uterine inflammatory response in mares submitted to frozen semen AI: 1. in the body of the uterus (AI) 2. by deep uterine insemination (DUI) or 3. by AI 24 hours after uterine infusion of SP and INRA96, The ovarian activity of 8 healthy cyclic Trotter mares was monitored by ultrasound (US) for 3 cycles. During each estrus at the evidence of a growing follicle ≥35mm ovulation was induced by 6.6 mg buserelin acetate (Suprefact®, Sanofi-Aventis, Milan, Italy; hour 0). Each cycle, mares were included in a different insemination group: • AI, • DUI , • Uterine infusion with 7.5 ml of equine SP + 15 ml of INRA96 24 hours before AI (UI+AI). All the mares were submitted to AI/DUI at hour 40 from induction, using 1 ml of frozen/thawed semen. Uterine inflammatory response (IR) was evaluated by US 6 and 20 hours after AI/DUI, and scored 0, 1 or 2 (no intrauterine fluid, fluid ≤2 cm or fluid >2 cm in height). Proportion and concentration of PMN was determined 6 hours after AI/DUI on a small volume uterine lavage performed with 60 ml of Lactated Ringer [5]. Pregnancies were diagnosed by embryo recovery (ER) at day 8 after ovulation. Differences between AI groups in embryo recovery rate were analysed by Fisher’s exact test. Differences in uterine score, % of PMN and PMN concentration ([PMN]) were analysed by repeated measures Friedman’s test and by Dunn’s Multiple Comparison as post-hoc test. Differences between AI groups for pregnancy rates and IR are shown in Tables 1 and 2. The removal of SP before stallion semen cryopreservation seems to reduce pregnancy rates in mares and it’s shown that SP could modulate post-mating induced endometritis [1-2]. The post-thaw addition of SP to frozen semen increased fertility [3,5] and modulated the PMN-influx into the uterine lumen [4,5] in mares and jennies. In the present study UI+AI mares, had a significantly lower [PMN] as previously described [2] even though there was a tendence of an higher uterine score 6 hours after AI/DUI. Pregnancy rates, however, where not improved compared to DUI and AI ones.
ADDITION OF SEMINAL PLASMA TO FROZEN SEMEN OR UTERINE INFUSION OF SEMINAL PLASMA AND INRA96 24 HOURS BEFORE INSEMINATION HAD NO EFFECT ON UTERINE INFLAMMATORY RESPONSE AND PREGNANCY RATE IN MARES
PANZANI, DUCCIO;CAMILLO, FRANCESCO;ROTA, ALESSANDRA
2015-01-01
Abstract
Removal of seminal plasma (SP) before cryopreservation or its addition after thawing affected pregnancy rates and uterine reaction after frozen semen artificial insemination [1-4]. Aims of this study were to evaluate the differences in pregnancy rates and uterine inflammatory response in mares submitted to frozen semen AI: 1. in the body of the uterus (AI) 2. by deep uterine insemination (DUI) or 3. by AI 24 hours after uterine infusion of SP and INRA96, The ovarian activity of 8 healthy cyclic Trotter mares was monitored by ultrasound (US) for 3 cycles. During each estrus at the evidence of a growing follicle ≥35mm ovulation was induced by 6.6 mg buserelin acetate (Suprefact®, Sanofi-Aventis, Milan, Italy; hour 0). Each cycle, mares were included in a different insemination group: • AI, • DUI , • Uterine infusion with 7.5 ml of equine SP + 15 ml of INRA96 24 hours before AI (UI+AI). All the mares were submitted to AI/DUI at hour 40 from induction, using 1 ml of frozen/thawed semen. Uterine inflammatory response (IR) was evaluated by US 6 and 20 hours after AI/DUI, and scored 0, 1 or 2 (no intrauterine fluid, fluid ≤2 cm or fluid >2 cm in height). Proportion and concentration of PMN was determined 6 hours after AI/DUI on a small volume uterine lavage performed with 60 ml of Lactated Ringer [5]. Pregnancies were diagnosed by embryo recovery (ER) at day 8 after ovulation. Differences between AI groups in embryo recovery rate were analysed by Fisher’s exact test. Differences in uterine score, % of PMN and PMN concentration ([PMN]) were analysed by repeated measures Friedman’s test and by Dunn’s Multiple Comparison as post-hoc test. Differences between AI groups for pregnancy rates and IR are shown in Tables 1 and 2. The removal of SP before stallion semen cryopreservation seems to reduce pregnancy rates in mares and it’s shown that SP could modulate post-mating induced endometritis [1-2]. The post-thaw addition of SP to frozen semen increased fertility [3,5] and modulated the PMN-influx into the uterine lumen [4,5] in mares and jennies. In the present study UI+AI mares, had a significantly lower [PMN] as previously described [2] even though there was a tendence of an higher uterine score 6 hours after AI/DUI. Pregnancy rates, however, where not improved compared to DUI and AI ones.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
