Paleonutrition and Paleopathology: Food and Disease at the Renaissance Courts of Naples and Florence

The study concerns 25 individuals from the Basilica of S. Domenico Maggiore in Naples (15th-17th centuries) and of 20 individuals from the Medici Chapels of the Basilica of S. Lorenzo in Florence (16th-17th centuries). The isotopes clearly reflect the large intake not only of meat but also of marine foods by the Italian aristocratic classes, especially from southern Italy, in the 15th-17th centuries. I present three important “clinical” cases. The left foot of Ferdinando I de’ Medici, Grand Duke of Tuscany (15491609), shows, at the peri-articular and articular surface of the interphalangeal joint of the hallux dorsum a lesion typical of chronic gout. High values of δ15N demonstrate a diet very rich in meat from terrestrial animals. This isotopic profile well correlates with the frequent attacks of gout referred by court chroniclers and with the diagnosis of chronic gout of the left big toe revealed by the paleopathological study. The skull of Don Filippino de’ Medici (1577-1582) shows nonsevere external hydrocephaly. The δ15N isotope values of don Filippino reveal a diet very rich in proteins of animal origin. The hereditary prince, was a frail and sickly child, affected by rickets. Probably for this reason, his parents and the court doctors forced him to eat more meat, considered at that time “the first source of physical strength”. Autopsy of the mummy of Ferrante I d’Aragona, king of Naples (1431-1494), revealed a moderately differentiated colon adenocarcinoma extensively infiltrating the muscles of the small pelvis. Ancient DNA amplification of the neoplastic cells by PCR in the mummy of Ferrante I evidenced a typical point mutation of the K-ras gene codon. The portraits of Ferrante reveal growing obesity from youth to maturity. Examination of the mummy of Ferrante, confirms his obesity. The paleonutritional data, with their high level of δ15N, show a massive intake of animal proteins. The alimentary “environment” of the Neapolitan court of the XV century and the sovereign’s habits, with his abundance of natural endogenous alkylating agents, well explain the K-ras mutation causing the tumor which killed the Aragonese king over five centuries ago.