La banca del tessuto osseo [The Tuscany bone bank]

Regarding anamnesis and serologic criteria for donor selection the Bank follows the dedicated guidelines of the Italian National Transplant Centre. The Bank selects the range of age (18-50 years) for ligament allograft and the evaluation protocol for detection of microbic contaminations (following international guidelines). In our protocol, each allograft has bacteriologic test for aerobic and anaerobic bacteria immediately at the moment of retrieval. When a careful review of the medical social history, and serologic tests shows eligibility of the tissue for transplant, the allograft is processed in a clean room at the appropriate size and x-ray are taken for future matching. After processing, but before antibiotic immersion allografts are again tested for microbiologic contamination, then packaged and taken in quarantine (-80 °C) freezer, waiting the results of bacteriologic tests: only if negative again, the allografts are released for transplantation. All the data concerning the donor and processing are kipped in an informatic database and subsequently are added all the information regarding the recipient, for traceability. We may dispose of supplementary processing techniques for an easier storage-room temperature (as freeze drying or sterilization program for contaminated allografts).