Bone marrow as a source of mesenchymal progenitors for the treatment of osteopenic defects has been introduced both in experimental models and, recently, in clinical practice. Under normal conditions, mesenchymal progenitors concentration in bone marrow is usually lower than 1% but they can be easily purified and expandeex vivo. The aim of this study is to evaluate the capability of minimally manipulated bone marrow cells to fasten bone regeneration following surgery for osteopenic defects. From June 2000 to August 2006, a total of 89 procedures were performed on 79 patients diagnosed with various osteopenic disorders (mainly bone cysts and non-unions) and who underwent bone marrow harvest from posterior iliac crests; a mean of 195 (75–285) mL were collected. Buffy coat was obtained in 38 cases by traditional procedure (centrifugation at 700 g for 10 min). The other 51 cases were processed by SEPAX (BIOSAFE, Switzerland), a centrifugation device for automated processing of blood components in a closed and sterile environment. Final results were a mean buffy-coat volume of 26 (7–56) mL, containing 2, 9 (0, 23–8, 9)109 nucleated cells (TNCs). Mesenchymal progenitors were evaluated for the presence of cells generating fibroblast-like colonies (CFU-F). The mean number of CFU-F obtained was 38 (5–227)/1106 TNC. Buffy-coat cells were implanted either directly in the bone defect site or after resuspension with bone matrix and autologous platelets followed by Ca11 in order to induce clotting and platelet degranulation. The procedure was well tolerated and no side effects were recorded. Bone regeneration was shown to be faster when compared with historical controls. Both prospective trials and animal models are currently under development.
Enhancing bone defects repair using autologous bone marrow buffy-coat
CAPANNA, RODOLFO;
2007-01-01
Abstract
Bone marrow as a source of mesenchymal progenitors for the treatment of osteopenic defects has been introduced both in experimental models and, recently, in clinical practice. Under normal conditions, mesenchymal progenitors concentration in bone marrow is usually lower than 1% but they can be easily purified and expandeex vivo. The aim of this study is to evaluate the capability of minimally manipulated bone marrow cells to fasten bone regeneration following surgery for osteopenic defects. From June 2000 to August 2006, a total of 89 procedures were performed on 79 patients diagnosed with various osteopenic disorders (mainly bone cysts and non-unions) and who underwent bone marrow harvest from posterior iliac crests; a mean of 195 (75–285) mL were collected. Buffy coat was obtained in 38 cases by traditional procedure (centrifugation at 700 g for 10 min). The other 51 cases were processed by SEPAX (BIOSAFE, Switzerland), a centrifugation device for automated processing of blood components in a closed and sterile environment. Final results were a mean buffy-coat volume of 26 (7–56) mL, containing 2, 9 (0, 23–8, 9)109 nucleated cells (TNCs). Mesenchymal progenitors were evaluated for the presence of cells generating fibroblast-like colonies (CFU-F). The mean number of CFU-F obtained was 38 (5–227)/1106 TNC. Buffy-coat cells were implanted either directly in the bone defect site or after resuspension with bone matrix and autologous platelets followed by Ca11 in order to induce clotting and platelet degranulation. The procedure was well tolerated and no side effects were recorded. Bone regeneration was shown to be faster when compared with historical controls. Both prospective trials and animal models are currently under development.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
