Particulate Matter (PM) is the atmospheric pollutant that mostly affects human health. One aim of the MAPEC_LIFE study is to evaluate children exposure to urban air pollution investigating the PM0.5 mutagenic and genotoxic effect. The samples were collected in children school areas in two seasons (winter-spring) using a high-volume air sampler. PM0.5 organic extracts were chemically analyzed, assayed on four Salmonella strains by Ames test and on A549 cell line by comet assay and micronucleus test. Results revealed that PM0.5 represents a very variable PM10 percentage (range 19.6-63% and 9.9-55.9% in winter and spring respectively). In winter all PM0.5 extracts showed at least one mutagenic dose with Salmonella TA98 strain suggesting the presence of indirect mutagens, while a lower effect was observed with the TA100 strain. The results with TA98NR and YG1021 strains in both seasons showed the presence of nitroaromatic compounds as confirmed by the chemical analysis. No genotoxic or oxidative effect was observed using the comet assay and micronucleus test in both seasons. The results suggest to investigate the biological effect of the other PM fractions, in particular PM0.5-1.
Evaluation of mutagenic/genotoxic effect of PM0.5 collected in five italian towns in two seasons: results of the MAPEC_LIFE study (LIFE12 ENV/IT/000614)
VERANI, MARCO;CARDUCCI, ANNALAURA;CASINI, BEATRICE;DONZELLI, GABRIELE;
2016-01-01
Abstract
Particulate Matter (PM) is the atmospheric pollutant that mostly affects human health. One aim of the MAPEC_LIFE study is to evaluate children exposure to urban air pollution investigating the PM0.5 mutagenic and genotoxic effect. The samples were collected in children school areas in two seasons (winter-spring) using a high-volume air sampler. PM0.5 organic extracts were chemically analyzed, assayed on four Salmonella strains by Ames test and on A549 cell line by comet assay and micronucleus test. Results revealed that PM0.5 represents a very variable PM10 percentage (range 19.6-63% and 9.9-55.9% in winter and spring respectively). In winter all PM0.5 extracts showed at least one mutagenic dose with Salmonella TA98 strain suggesting the presence of indirect mutagens, while a lower effect was observed with the TA100 strain. The results with TA98NR and YG1021 strains in both seasons showed the presence of nitroaromatic compounds as confirmed by the chemical analysis. No genotoxic or oxidative effect was observed using the comet assay and micronucleus test in both seasons. The results suggest to investigate the biological effect of the other PM fractions, in particular PM0.5-1.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
