The model protein hen egg white lysozyme was challenged with Oxaliplatin and Cisplatin. ESI mass spectrometry, surface plasmon resonance and thermal shift analyses demonstrate the formation of a bis-platinum adduct, though in very small amounts. Crystals of the bis-platinum adduct were obtained using two different preparations and the X-ray structures solved at 1.85 Ǻ and 1.95 Ǻ resolution. Overall, the obtained data point out that, under the analyzed conditions, the two Pt drugs have similar affinities for the protein, but bind on its surface at two non-overlapping sites. In other words, these two drugs manifest a significantly different reactivity with this model protein and do not compete for the same protein binding sites.
Oxaliplatin vs. cisplatin: Competition experiments on their binding to lysozyme
MARZO, TIZIANOPenultimo
;
2015-01-01
Abstract
The model protein hen egg white lysozyme was challenged with Oxaliplatin and Cisplatin. ESI mass spectrometry, surface plasmon resonance and thermal shift analyses demonstrate the formation of a bis-platinum adduct, though in very small amounts. Crystals of the bis-platinum adduct were obtained using two different preparations and the X-ray structures solved at 1.85 Ǻ and 1.95 Ǻ resolution. Overall, the obtained data point out that, under the analyzed conditions, the two Pt drugs have similar affinities for the protein, but bind on its surface at two non-overlapping sites. In other words, these two drugs manifest a significantly different reactivity with this model protein and do not compete for the same protein binding sites.File | Dimensione | Formato | |
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