Objectives: The aim of this study was to evaluate the analgesic and the anti-inflammatory activity of Opuntia microdasys at post flowering stage, F3 (OMF3) in rat and, in other hand, its antigenotoxic effects by the Allium cepa test. Methods: OMF3 extracts were screened for activity of analgesic and anti-inflammatory using, respectively, the acetic acid writhing test in mice and the carrageenan-induced paw oedema assay in rats. The antigenotoxic has been evaluated by A. cepa test. Key findings: OMF3 extracts showed a higher analgesic and anti-inflammatory activity at 100 mg/kg (72.03% and 70.11%) as determined by the tests of acetic acid-induced writhing and carrageenan-induced oedema, respectively. Furthermore, the OMF3 aqueous extracts have a preventive antimutagenic potential, at lower concentration (EC50 ≈ 60 μg/ml), against H2O2-mediated DNA damage in A. cepa root meristem cells with an efficient restoration of the mitotic index in comparison with controls. Conclusions: Based on this study, the flower of O. microdasys at post flowering stage may be use as an analgesic, anti-inflammatory and antimutagenic agents.

Activity of anti-inflammatory, analgesic and antigenotoxic of the aqueous flower extracts of Opuntia microdasys Lem.Pfeiff

FLAMINI, GUIDO
Penultimo
;
2017

Abstract

Objectives: The aim of this study was to evaluate the analgesic and the anti-inflammatory activity of Opuntia microdasys at post flowering stage, F3 (OMF3) in rat and, in other hand, its antigenotoxic effects by the Allium cepa test. Methods: OMF3 extracts were screened for activity of analgesic and anti-inflammatory using, respectively, the acetic acid writhing test in mice and the carrageenan-induced paw oedema assay in rats. The antigenotoxic has been evaluated by A. cepa test. Key findings: OMF3 extracts showed a higher analgesic and anti-inflammatory activity at 100 mg/kg (72.03% and 70.11%) as determined by the tests of acetic acid-induced writhing and carrageenan-induced oedema, respectively. Furthermore, the OMF3 aqueous extracts have a preventive antimutagenic potential, at lower concentration (EC50 ≈ 60 μg/ml), against H2O2-mediated DNA damage in A. cepa root meristem cells with an efficient restoration of the mitotic index in comparison with controls. Conclusions: Based on this study, the flower of O. microdasys at post flowering stage may be use as an analgesic, anti-inflammatory and antimutagenic agents.
Chahdoura, Hassiba; El Bok, Safia; Refifa, Taoufik; Adouni, Khaoula; Khemiss, Fethia; Mosbah, Habib; Ben Attia, Mossadok; Flamini, Guido; Achour, Lotfi
File in questo prodotto:
File Dimensione Formato  
Opuntia microdasys antigenotoxic-JPP 2017.pdf

solo utenti autorizzati

Descrizione: articolo
Tipologia: Versione finale editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 463.29 kB
Formato Adobe PDF
463.29 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/856593
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 5
social impact