This study was aimed at evaluating the influence of sampling procedure on the determination of uric acid and lactate in oral fluid. Samples of non-stimulated and stimulated oral fluid were collected from 22 healthy volunteers. Different frequencies of stimulation were obtained by moving a polyester swab within the mouth at 50, 100 and 150 min–1. Three oral fluid samples were consecutively collected from a subgroup of 5 volunteers at a constant stimulation (70 min–1) and at a self-selected pace to evaluate reproducibility. The urate concentration in oral fluid decreased with the increase of the stimulation and oral fluid flow rate (r = -0.98, p = 0.01). Also, the lactate concentration was much (p = 0.03, two tailed) lower in samples collected under a mild stimulation (50 min–1) than in samples collected without stimulation. Nevertheless, it progressively increased at higher stimulations (100 and 150 min–1). A transfer process mediated by membrane carriers (i.e. urate transporter and organic anion transporters) was hypothesized to explain these results. Finally, a reduced variability (relative standard deviation below 10%) of the urate concentration was obtained when oral fluid was sampled at constant stimulation (70 min–1), but it increased remarkably (20–50%) in case of sampling at self-selected pace. Nevertheless, expressing the salivary excretion of urate as a function of time (μgmin–1), the variability of sampling procedure at self-selected pace was lower than 15%.

The effect of sampling procedures on the urate and lactate concentration in oral fluid

LOMONACO, TOMMASO;GHIMENTI, SILVIA;BIAGINI, DENISE;BELLAGAMBI, FRANCESCA;FUOCO, ROGER;DI FRANCESCO, FABIO
2018-01-01

Abstract

This study was aimed at evaluating the influence of sampling procedure on the determination of uric acid and lactate in oral fluid. Samples of non-stimulated and stimulated oral fluid were collected from 22 healthy volunteers. Different frequencies of stimulation were obtained by moving a polyester swab within the mouth at 50, 100 and 150 min–1. Three oral fluid samples were consecutively collected from a subgroup of 5 volunteers at a constant stimulation (70 min–1) and at a self-selected pace to evaluate reproducibility. The urate concentration in oral fluid decreased with the increase of the stimulation and oral fluid flow rate (r = -0.98, p = 0.01). Also, the lactate concentration was much (p = 0.03, two tailed) lower in samples collected under a mild stimulation (50 min–1) than in samples collected without stimulation. Nevertheless, it progressively increased at higher stimulations (100 and 150 min–1). A transfer process mediated by membrane carriers (i.e. urate transporter and organic anion transporters) was hypothesized to explain these results. Finally, a reduced variability (relative standard deviation below 10%) of the urate concentration was obtained when oral fluid was sampled at constant stimulation (70 min–1), but it increased remarkably (20–50%) in case of sampling at self-selected pace. Nevertheless, expressing the salivary excretion of urate as a function of time (μgmin–1), the variability of sampling procedure at self-selected pace was lower than 15%.
2018
Lomonaco, Tommaso; Ghimenti, Silvia; Biagini, Denise; Bramanti, E.; Onor, Massimo; Bellagambi, Francesca; Fuoco, Roger; DI FRANCESCO, Fabio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/859628
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