Current knowledge of the beneﬁts of nutrition supplements for eye pathologies is based largely on the use of appropriate animal models, together with deﬁned dietary supplementation. Here, C57BL6 mice were subretinally injected with polyethylene glycol (PEG)-400, an established model of retinal degeneration with a dry age-related macular degeneration (AMD)-like phenotype, an eye pathology that lacks treatment. In response to PEG-400, markers of the complement system, angiogenesis,inﬂammation,gliosis,andmacrophageinﬁltrationwereupregulatedinbothretinasand retinal pigment epithelium (RPE)/choroids, whereas dietary supplementation with a mixture based on fatty acids counteracted their upregulation. Major effects include a reduction of inﬂammation, in both retinas and RPE/choroids, and an inhibition of macrophage inﬁltration in the choroid, yet not in the retina, suggesting a targeted action through the choroidal vasculature. Histological analysis revealed a thinning of the outer nuclear layer (ONL), together with dysregulation of the epithelium layer in response to PEG-400. In addition, immunohistoﬂuorescence demonstrated Müller cell gliosis and macrophage inﬁltration into subretinal tissues supporting the molecular ﬁndings. Reduced ONL thickness,gliosis,andmacrophageinﬁltrationwerecounteractedbythedietsupplement. The present data suggest that fatty acids may represent a useful form of diet supplementation to prevent or limit the progression of dry AMD.
CAMMALLERI, MAURIZIO (Co-primo)
DAL MONTE, MASSIMO (Co-primo)
|Autori:||Cammalleri, Maurizio; DAL MONTE, Massimo; Locri, Filippo; Lardner, E.; Kvanta, A.; Rusciano, D.; André, H.; Bagnoli, Paola|
|Titolo:||Efficacy of fatty acids dietary supplement in polyethylene glycol-induced mouse model of retinal degeneration|
|Anno del prodotto:||2017|
|Digital Object Identifier (DOI):||10.3390/nu9101079|
|Appare nelle tipologie:||1.1 Articolo in rivista|