In vitro grown quince (Cydonia oblonga Mill) BA 29 leaves were cultured for 2 days in liquid MS medium containing 2,4-D (11.3 µM). The subsequent gelled MS medium contained Kinetin (4.7 µM) and NAA (0.5 µM). The effects of three induction temperatures (23, 28, 33 ˚C) during 2,4-D induction treatment and six temperatures (19, 21, 23, 25, 28, 33 ˚C) during morphogenic structures development were evaluated. Moreover the effects of different shocks with low (0, -5, -10, -15 ˚C) or high temperatures (35, 40, 45, 50 and 60 ˚C) applied for different time (1, 2, 4, 6, 8, 24 hours) during the induction stage were compared. Percentage of regenerating leaves and number of somatic embryos and adventitious roots was recorded. The different temperatures influenced both somatic embryo and root formation. In both the induction and development stage, 23 ˚C proved to be the best temperature for embryo production, while 21 ˚C applied during the development period appeared to be better for root formation. The short induction temperature treatments (1 hour) further enhanced embryo and root formation. Among the low temperatures, -10 ˚C was the most successful for both morphogenic processes, while among high temperatures the greatest number of morphogenic formations was observed at 40; in the latter conditions morphogenesis was higher than with low temperature. The most satisfactory treatment length for the 40 ˚C short temperature treatment was 1-4 hours for somatic embryo formation and 1-2 hour for adventitious root production. These results suggested that a short shock at 40 ˚C, applied during induction performed at 23 ˚C, in combination with 21-23 ˚C during the development stage, is the best temperature combination for in vitro quince somatic embryogenesis.
Effect of high and low temperature on the leaf regenerating capacity of quince BA29 rootstock
MORINI, STEFANO;D'ONOFRIO, CLAUDIO;LORETI, FILIBERTO
2004-01-01
Abstract
In vitro grown quince (Cydonia oblonga Mill) BA 29 leaves were cultured for 2 days in liquid MS medium containing 2,4-D (11.3 µM). The subsequent gelled MS medium contained Kinetin (4.7 µM) and NAA (0.5 µM). The effects of three induction temperatures (23, 28, 33 ˚C) during 2,4-D induction treatment and six temperatures (19, 21, 23, 25, 28, 33 ˚C) during morphogenic structures development were evaluated. Moreover the effects of different shocks with low (0, -5, -10, -15 ˚C) or high temperatures (35, 40, 45, 50 and 60 ˚C) applied for different time (1, 2, 4, 6, 8, 24 hours) during the induction stage were compared. Percentage of regenerating leaves and number of somatic embryos and adventitious roots was recorded. The different temperatures influenced both somatic embryo and root formation. In both the induction and development stage, 23 ˚C proved to be the best temperature for embryo production, while 21 ˚C applied during the development period appeared to be better for root formation. The short induction temperature treatments (1 hour) further enhanced embryo and root formation. Among the low temperatures, -10 ˚C was the most successful for both morphogenic processes, while among high temperatures the greatest number of morphogenic formations was observed at 40; in the latter conditions morphogenesis was higher than with low temperature. The most satisfactory treatment length for the 40 ˚C short temperature treatment was 1-4 hours for somatic embryo formation and 1-2 hour for adventitious root production. These results suggested that a short shock at 40 ˚C, applied during induction performed at 23 ˚C, in combination with 21-23 ˚C during the development stage, is the best temperature combination for in vitro quince somatic embryogenesis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
