The SPOT synthesis is a novel technique for the simultaneous multiple synthesis of peptides covalently linked to cellulose paper, using conventional solid-phase synthesis strategy. The sets of paper-bound peptides thus obtained can be directly used in solid-phase assays, including ELISA. The technique is a powerful tool for the study of protein-interactions and has been widely applied to antibody characterization and epitope mapping studies. The SPOT technique provides a simple, economic and rapid access to large numbers of short peptide sequences or peptide mixtures which can be tested concomitantly with a single serum and repeatedly with many sera on the same membrane. Recent applications of SPOT synthesis to epitope mapping are reviewed in this report.
Spot-Synthesis: Easy Multiple Preparation of Synthetic Peptides on Cellulose Membrane for the Analysis of Anti-Protein Antibodies
Lucchesi Daniela;
1997-01-01
Abstract
The SPOT synthesis is a novel technique for the simultaneous multiple synthesis of peptides covalently linked to cellulose paper, using conventional solid-phase synthesis strategy. The sets of paper-bound peptides thus obtained can be directly used in solid-phase assays, including ELISA. The technique is a powerful tool for the study of protein-interactions and has been widely applied to antibody characterization and epitope mapping studies. The SPOT technique provides a simple, economic and rapid access to large numbers of short peptide sequences or peptide mixtures which can be tested concomitantly with a single serum and repeatedly with many sera on the same membrane. Recent applications of SPOT synthesis to epitope mapping are reviewed in this report.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
