The temporal and spatial distribution of the auxin is determined by the polar subcellular localization of PIN-FORMED (PIN) auxin efflux carrier family. Phosphorylation dynamics of PIN proteins are affected by the protein phosphatase 2A and the Ser/Thr protein kinase PINOID (PID). A PID-LIKE (Ha-PIDL) gene has been isolated in sunflower also to perform a candidate-gene approach for the mutant missing flowers (mf). Sequence and phylogenetic analyses suggested that Ha-PIDL was the orthologs of the PID transcript. Real-Time Quantitative Reverse Transcription PCR analysis revealed a high accumulation of Ha-PIDL transcripts in vegetative shoots and inflorescence shoots of sunflower. In addition, sequence and transcriptional analyses suggested that the phenotype of the mf mutant was not due to a mutation and/or altered transcription of Ha-PIDL. Nevertheless, the isolation and characterization of a PID-LIKE gene should be critical to study the polar auxin transport in
Sequence analysis and expression profile of a PINOID-LIKE gene in organs of sunflower (Helianthus annuus L.) and in the missing flowers mutant
FAMBRINI M.Primo
;PUGLIESI C.
2017-01-01
Abstract
The temporal and spatial distribution of the auxin is determined by the polar subcellular localization of PIN-FORMED (PIN) auxin efflux carrier family. Phosphorylation dynamics of PIN proteins are affected by the protein phosphatase 2A and the Ser/Thr protein kinase PINOID (PID). A PID-LIKE (Ha-PIDL) gene has been isolated in sunflower also to perform a candidate-gene approach for the mutant missing flowers (mf). Sequence and phylogenetic analyses suggested that Ha-PIDL was the orthologs of the PID transcript. Real-Time Quantitative Reverse Transcription PCR analysis revealed a high accumulation of Ha-PIDL transcripts in vegetative shoots and inflorescence shoots of sunflower. In addition, sequence and transcriptional analyses suggested that the phenotype of the mf mutant was not due to a mutation and/or altered transcription of Ha-PIDL. Nevertheless, the isolation and characterization of a PID-LIKE gene should be critical to study the polar auxin transport inI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.