We have modified the biolistic process to establish a transient assay for rapid analysis of transcription factors in arabidopsis. ATHB-1, an arabidopsis HD-Zip protein, was used as a transacting factor in particle bombardment experiments with arabidopsis leaves, and was found to transactivate a reporter gene in a binding site-specific manner. The experiments show the feasibility of using microprojectile-mediated gene transfer for rapid functional analysis of transcription factors in arabidopsis.

A transient assay for rapid functional analysis of transcription factors in arabidopsis

Borello U;
1998

Abstract

We have modified the biolistic process to establish a transient assay for rapid analysis of transcription factors in arabidopsis. ATHB-1, an arabidopsis HD-Zip protein, was used as a transacting factor in particle bombardment experiments with arabidopsis leaves, and was found to transactivate a reporter gene in a binding site-specific manner. The experiments show the feasibility of using microprojectile-mediated gene transfer for rapid functional analysis of transcription factors in arabidopsis.
Sessa, G; Borello, U; Morelli, G; Ruberti, I
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/932973
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