A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy. RI Bizzarri, Ranieri/H-1779-2011
Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging
Bizzarri R
2012-01-01
Abstract
A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy. RI Bizzarri, Ranieri/H-1779-2011File in questo prodotto:
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