A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy. RI Bizzarri, Ranieri/H-1779-2011

Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

Bizzarri R
2012-01-01

Abstract

A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy. RI Bizzarri, Ranieri/H-1779-2011
2012
Battisti, A; Digman, Ma; Gratton, E; Storti, B; Beltram, F; Bizzarri, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/991383
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