Natural products play a significant role in the drug discovery for the treatment of human diseases. A large number of bioactive compounds is produced by plants and among studied plants about 20% have been used for pharmaceutical purposes [1]. Moreover, the consumption of fruits and vegetables is highly recommended due to their medicinal properties and high nutritional value [2]. For this reason, a rapid detection of biologically active substances plays a key role in the chemical investigation of phyto-preparations. Crude plant extracts are very complex mixtures and the isolation process is a time consuming task [3]. The development of techniques such as high performance liquid chromatography coupled to photodiode array/UV (LC-PDA/UV) and to mass spectrometer (LC-MS or LC-MS/MS) has provided highly selective and sensitive methods for the chemical analysis of complex herbal mixtures, by allowing a rapid structural determination of known plant constituents with only a low amount of sample [4]. In this talk, the chemical characterization of phyto-preparations from several Mediterranean medicinal and edible plants, showing interesting biological activities, is presented, with particular regard to their phenolic content. Analyses were performed using a LC-PDA/UV-MS system and the electrospray ionization (ESI) technique combined with multi-stage tandem mass spectrometry (MSn). With the acquisition of complementary spectral and chromatographic data on detected components, the major constituents of studied herbal mixtures were identified providing the chemical profiles of biological active extracts. 1. Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod 2016; 79:629-61. 2. Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants (Basel) 2017;6:42. 3. Wolfender JL, Terreaux C, Hostettmann K. The importance of LC-MS and LC-NMR in the discovery of new lead compounds from plants. Pharm Biol 2000;38:41-54. 4. Steinmann D, Ganzera M. Recent advances on HPLC/MS in medicinal plant analysis. J Pharm Biomed Anal 2011;55:744-57.
LC-ESI-MS/MS as a tool for chemical characterization of edible and medicinal plants
De Leo Marinella
2019-01-01
Abstract
Natural products play a significant role in the drug discovery for the treatment of human diseases. A large number of bioactive compounds is produced by plants and among studied plants about 20% have been used for pharmaceutical purposes [1]. Moreover, the consumption of fruits and vegetables is highly recommended due to their medicinal properties and high nutritional value [2]. For this reason, a rapid detection of biologically active substances plays a key role in the chemical investigation of phyto-preparations. Crude plant extracts are very complex mixtures and the isolation process is a time consuming task [3]. The development of techniques such as high performance liquid chromatography coupled to photodiode array/UV (LC-PDA/UV) and to mass spectrometer (LC-MS or LC-MS/MS) has provided highly selective and sensitive methods for the chemical analysis of complex herbal mixtures, by allowing a rapid structural determination of known plant constituents with only a low amount of sample [4]. In this talk, the chemical characterization of phyto-preparations from several Mediterranean medicinal and edible plants, showing interesting biological activities, is presented, with particular regard to their phenolic content. Analyses were performed using a LC-PDA/UV-MS system and the electrospray ionization (ESI) technique combined with multi-stage tandem mass spectrometry (MSn). With the acquisition of complementary spectral and chromatographic data on detected components, the major constituents of studied herbal mixtures were identified providing the chemical profiles of biological active extracts. 1. Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod 2016; 79:629-61. 2. Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants (Basel) 2017;6:42. 3. Wolfender JL, Terreaux C, Hostettmann K. The importance of LC-MS and LC-NMR in the discovery of new lead compounds from plants. Pharm Biol 2000;38:41-54. 4. Steinmann D, Ganzera M. Recent advances on HPLC/MS in medicinal plant analysis. J Pharm Biomed Anal 2011;55:744-57.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
