This paper describes the design of a chip modeling the proximal renal tubule, namely the nephron segment that is most targeted by drug toxicants. The tubule model is based on a 3D convoluted geometry whose printing parameters and Pluronic concentration were optimized to achieve a diameter of $320~mu ext{m}$ without discontinuities. A perfusion system guaranteeing a $1~mu ext{L}$ /min flow was also integrated. Moreover, the tubule model was integrated into a gel recapitulating the composition and the elastic properties of the human kidney extracellular matrix. Different formulations containing Matrigel, Collagen I and transglutaminase were investigated and compared in terms of rheometric properties and renal cell viability and ability to form a compact layer on them. Optimal results were found with a matrix composed of Matrigel and Collagen I (especially in the ratios 1:1), with the addition of 2.7% transglutaminase and 0.9% CaCl2.

3D Printed Perfusable Renal Proximal Tubule Model with Different Extracellular Matrix Compositions

Mazzeo A.;Iacovacci V.;Riacci L.;Trucco D.;Vistoli F.;Ricotti L.
2021

Abstract

This paper describes the design of a chip modeling the proximal renal tubule, namely the nephron segment that is most targeted by drug toxicants. The tubule model is based on a 3D convoluted geometry whose printing parameters and Pluronic concentration were optimized to achieve a diameter of $320~mu ext{m}$ without discontinuities. A perfusion system guaranteeing a $1~mu ext{L}$ /min flow was also integrated. Moreover, the tubule model was integrated into a gel recapitulating the composition and the elastic properties of the human kidney extracellular matrix. Different formulations containing Matrigel, Collagen I and transglutaminase were investigated and compared in terms of rheometric properties and renal cell viability and ability to form a compact layer on them. Optimal results were found with a matrix composed of Matrigel and Collagen I (especially in the ratios 1:1), with the addition of 2.7% transglutaminase and 0.9% CaCl2.
Mazzeo, A.; Iacovacci, V.; Riacci, L.; Trucco, D.; Lisignoli, G.; Vistoli, F.; Ricotti, L.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/1137391
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