Cytotoxic sesquiterpenoids from Ammoides atlantica aerial parts

Seven new terpenoids, four of the guaiane-type (1-4), an eudesmane (5), and a bisabolane (6) sesquiterpenoids, and a furanone (7), were isolated from the aerial parts of Ammoides atlantica, a herbaceous plant growing in Algeria, together with eight known compounds. All metabolites were characterized by their 1D, 2D NMR, and HRESIMS data. A combined DFT/NMR method was applied to study the relative configurations of 1-4, 6, and 7. All compounds, except 2, were assayed against MCF-7, A375, A549, HaCaT, and Jurkat cell lines. Compounds 8, 10, and 11 induced a dose-dependent reduction in cell viability with different potency on almost all cell lines used. The most active compounds 8 and 10 were studied to assess their potential apoptotic effects and cell cycle inhibition.