Owing to the traditional phytotherapeutic use of basil and its importance as basic component of the Mediterranean diet, this research aimed to study the nutraceutical properties of basil (Ocimum basilicum cv. Genova) grown in hydroponics in comparison with that grown in soil. The antioxidant activity of aqueous and lipid extracts of basil leaves was evaluated both by spectrophotometric detection with the 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+) and by Electron Paramagnetic Resonance (EPR) detection with the stable radicals peroxylamine disulphonate (Fremy’s salt, hydrophilic) and 1,1-diphenyl-2-picrylhydrazyl (DPPH, lipophilic). From EPR decay kinetics analysis it was possible to distinguish in the lipid extract a fast rate constant and a slow rate constant, likely attributable to two different kind of lipophilic antioxidants. Hydroponic cultivation improved antioxidant activity of both aqueous and lipid extracts increasing the contents in vitamin C, vitamin E, lipoic acid, total phenols as well as in rosmarinic acid.
Qualità nutraceutiche di piante coltivate a terra od in idroponica: EPR come metodo di indagine del potere antiradicalico
IZZO, RICCARDO;SGHERRI, CRISTINAWriting – Original Draft Preparation
;
2008-01-01
Abstract
Owing to the traditional phytotherapeutic use of basil and its importance as basic component of the Mediterranean diet, this research aimed to study the nutraceutical properties of basil (Ocimum basilicum cv. Genova) grown in hydroponics in comparison with that grown in soil. The antioxidant activity of aqueous and lipid extracts of basil leaves was evaluated both by spectrophotometric detection with the 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+) and by Electron Paramagnetic Resonance (EPR) detection with the stable radicals peroxylamine disulphonate (Fremy’s salt, hydrophilic) and 1,1-diphenyl-2-picrylhydrazyl (DPPH, lipophilic). From EPR decay kinetics analysis it was possible to distinguish in the lipid extract a fast rate constant and a slow rate constant, likely attributable to two different kind of lipophilic antioxidants. Hydroponic cultivation improved antioxidant activity of both aqueous and lipid extracts increasing the contents in vitamin C, vitamin E, lipoic acid, total phenols as well as in rosmarinic acid.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
