A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Gli1 expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.
|Autori:||Gallo, R; Grieco, Fa; Marselli, Lorella; Ferretti, E; Gulino, A; Marchetti, Piero; Dotta, F.|
|Titolo:||Hedgehog signaling during expansion of human pancreatic islet-derived precursors|
|Anno del prodotto:||2008|
|Digital Object Identifier (DOI):||10.1196/annals.1447.024|
|Appare nelle tipologie:||1.1 Articolo in rivista|