In vitro and in vivo antimicrobial activity of medicated wipes containing biosecur on the microbial Flora of the eyelid and conjunctiva

: PurposeTo evaluate the in vitro and in vivo antimicrobial activity on the microbial flora of the eyelids and conjunctiva of the new medicated wipes LENIVA bio, soaked with an antiseptic solution containing natural extracts from citrus fruits (Biosecur®), Aloe vera, and Ruscus aculeatus.MethodsThe in vitro antimicrobial activity of the LENIVA bio antiseptic solution against bacteria and fungi commonly found in the periocular area, including antibiotic-resistant strains, was measured by agar diffusion and microdilution assays. The in vivo activity was measured on swabs collected from the eyelid rim and conjunctiva of 20 patients (13 males, 7 females; mean age 70.5 years) before and after a 4-day treatment with the product. Contralateral untreated eyes were used as controls.ResultsThe in vitro analysis of inhibition halos in agar diffusion assays demonstrated that LENIVA bio is active against all tested germs, even when diluted. In vivo, the reduction in the total microbial counts obtained from both eyelid rim and conjunctival swabs following treatment with LENIVA bio wipes was statistically significant (respectively, mean reduction = 4530 CFU/ml, p < 0.0001; mean reduction = 893 CFU/ml, p = 0.0384).ConclusionLENIVA bio wipes showed in vitro and in vivo antimicrobial activity against microorganisms that are potential pathogens of the eye and/or commonly found in the periocular area.