In the frame of the project NUTRASNACK (E.C. F.P.6 contract No FOOD-CT-2005-023044) which has the main objective to increase the antioxidant potential of food snacks, several Salvia species and genotypes have been tested for their total antioxidant capability. Three genotypes with the highest antioxidant values were selected (S. officinalis “SOF1”, S. officinalis “SOF2” and S. officinalis “SOF3”) and leaf explants of in vivo grown plants were cultured onto modified agarised MS media supplemented with 2,4-D, Kinetin and NAA in several combinations in order to induce friable callus. Friable green callus developed after 5 weeks in the presence of the best medium containing 0.5 mgL-1 2,4-D and 0.5 mgL-1 Kinetin, it was transferred several time in the same medium and after 3 months liquid primary cultures were established. After filtration the synchronized cells grew rapidly and the growth parameters such as viability, growth curve in dry and fresh weight gave the essential information for the management of the cultures. The EC 50 of total antioxidant capacity was evaluated during all the growth phases in calli and suspension cultures with DPPH test.
Establishment of in vitro Salvia cell biomass for the controlled production of antioxidant metabolites
BERTOLI, ALESSANDRA;PISTELLI, LUISA
2010-01-01
Abstract
In the frame of the project NUTRASNACK (E.C. F.P.6 contract No FOOD-CT-2005-023044) which has the main objective to increase the antioxidant potential of food snacks, several Salvia species and genotypes have been tested for their total antioxidant capability. Three genotypes with the highest antioxidant values were selected (S. officinalis “SOF1”, S. officinalis “SOF2” and S. officinalis “SOF3”) and leaf explants of in vivo grown plants were cultured onto modified agarised MS media supplemented with 2,4-D, Kinetin and NAA in several combinations in order to induce friable callus. Friable green callus developed after 5 weeks in the presence of the best medium containing 0.5 mgL-1 2,4-D and 0.5 mgL-1 Kinetin, it was transferred several time in the same medium and after 3 months liquid primary cultures were established. After filtration the synchronized cells grew rapidly and the growth parameters such as viability, growth curve in dry and fresh weight gave the essential information for the management of the cultures. The EC 50 of total antioxidant capacity was evaluated during all the growth phases in calli and suspension cultures with DPPH test.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
