The aim of this study was to provide a preliminary contribute in describing the expression profile of water-soluble proteinases of digestive gland of adult octopus at different pH and temperature conditions. The octopus (Octopus vulgaris Cuvier, 1797) were caught as sub—adult and randomly distributed in two groups. Broodstock was fed the same diet but the experimental group was boosted with 3 “crab tablets” per crab and 2 “sardine tablets” per sardine supplied. Tablets were formulated in function of crab, sardine octopus composition and its “supposed” nutritional requirements. Octopus digestive glands of females of both groups (5 for control, 3 for experimental) were sampled at the end of June and frozen at -20 °C until used for analysis. Zymograms of the octopus digestive gland, shows mainly 7 detectable activity bands ranging between 97 and 20kDa. The animals fed with tablets clearly show lower proteinases activity of both band 30 and 20kDa at ranges between 2 and 5 pH and 9-11 pH. In conclusion, zymography seems to be a useful tool to study the effect of different diets on octopus digestive proteinases
EXPRESSION PROFILE OF WATER–SOLUBLE PROTEINASES OF Octopus vulgaris Cuvier, 1797 INVESTIGATED BY ZYMOGRAPHY, FOR A NEW APPROACH IN NUTRITIONAL REQUIREMENT DETERMINATION
SAGONA, SIMONA;FELICIOLI, ANTONIO;FRONTE, BALDASSARE
2011-01-01
Abstract
The aim of this study was to provide a preliminary contribute in describing the expression profile of water-soluble proteinases of digestive gland of adult octopus at different pH and temperature conditions. The octopus (Octopus vulgaris Cuvier, 1797) were caught as sub—adult and randomly distributed in two groups. Broodstock was fed the same diet but the experimental group was boosted with 3 “crab tablets” per crab and 2 “sardine tablets” per sardine supplied. Tablets were formulated in function of crab, sardine octopus composition and its “supposed” nutritional requirements. Octopus digestive glands of females of both groups (5 for control, 3 for experimental) were sampled at the end of June and frozen at -20 °C until used for analysis. Zymograms of the octopus digestive gland, shows mainly 7 detectable activity bands ranging between 97 and 20kDa. The animals fed with tablets clearly show lower proteinases activity of both band 30 and 20kDa at ranges between 2 and 5 pH and 9-11 pH. In conclusion, zymography seems to be a useful tool to study the effect of different diets on octopus digestive proteinasesI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
