New fluorescent ligands for adenosine receptors are described; these compounds were obtained by the insertion, in the N-6 position of NECA (a potent adenosine agonist), of dansylaminoalkyl moieties with alkyl spacers of increasing carbon chain length (from 3 to 12). Among them, the compound with a C6 alkyl spacer proved to be the most interesting one, showing a marked selectivity for the A(1) receptor subtype; furthermore, in fluorescence microscopy assays it proved to be able to visualize and localize this receptor subtype at the level of the molecular layer of the rat cerebellar cortex. (C) 1998 Elsevier Science Ltd. All rights reserved.
Fluorescent probes for adenosine receptors: synthesis and biology of N6-dansylaminoalkyl-substituted NECA derivates
MACCHIA, MARCO;GESI, MARCO;LUCACCHINI, ANTONIO;SOLDANI, PAOLA;MARTINI, CLAUDIA
1998-01-01
Abstract
New fluorescent ligands for adenosine receptors are described; these compounds were obtained by the insertion, in the N-6 position of NECA (a potent adenosine agonist), of dansylaminoalkyl moieties with alkyl spacers of increasing carbon chain length (from 3 to 12). Among them, the compound with a C6 alkyl spacer proved to be the most interesting one, showing a marked selectivity for the A(1) receptor subtype; furthermore, in fluorescence microscopy assays it proved to be able to visualize and localize this receptor subtype at the level of the molecular layer of the rat cerebellar cortex. (C) 1998 Elsevier Science Ltd. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
