The thermodynamic functions, compressibility, entropy and free energy of desorption have been derived from a set of π-A isotherms of human immunoglobulin G films at an air-water interface. Criteria for optimum filmlift conditions have been obtained from an analysis of these functions. By the application of a basic model, a picture of the desorption behaviour of segments of the antibodies has also been constructed. The results of this study have been used to estimate the theoretical sensing limit of a TIRF based immunosensor.
Autori interni: | |
Autori: | A. Ahluwalia; De Rossi D.; M. Monici; A. Schirone |
Titolo: | Thermodynamic study of langmuir antibody films for application to immunosensors |
Anno del prodotto: | 1991 |
Digital Object Identifier (DOI): | 10.1016/0956-5663(91)87037-C |
Appare nelle tipologie: | 1.1 Articolo in rivista |
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