The thermodynamic functions, compressibility, entropy and free energy of desorption have been derived from a set of π-A isotherms of human immunoglobulin G films at an air-water interface. Criteria for optimum filmlift conditions have been obtained from an analysis of these functions. By the application of a basic model, a picture of the desorption behaviour of segments of the antibodies has also been constructed. The results of this study have been used to estimate the theoretical sensing limit of a TIRF based immunosensor.
|Autori:||A. Ahluwalia; De Rossi D.; M. Monici; A. Schirone|
|Titolo:||Thermodynamic study of langmuir antibody films for application to immunosensors|
|Anno del prodotto:||1991|
|Digital Object Identifier (DOI):||10.1016/0956-5663(91)87037-C|
|Appare nelle tipologie:||1.1 Articolo in rivista|