The micronucleus (MN) assay in peripheral blood lymphocytes was applied to assess the genotoxic potential of a single dose of iodine-131 () given to six patients for ablation of thyroid remnants after total thyroidectomy. Lymphocytes were taken at various times after therapy (from 2 to 180 days), and evaluated for the presence of MN in the binucleated cells identified after blocking cytokynesis with cytochalasin B. The presence of ultrafiltered, low-molecular weight, clastogenic factor(s) (CFs) in the plasma of 11 patient undergoing therapy was also sequentially assessed. A significantly increased MN frequency was observed in lymphocytes of patients as soon as the first sampling time (2 days after therapy), multifactor analysis of variance (MANOVA): P<0.0001, peaking at day 7 at almost four-fold the spontaneous frequency observed in the pre-therapy samples. MN frequency slowly declined thereafter, reaching the baseline levels at the 180-day time point. When tested against peripheral blood lymphocytes from a healthy donor, the ultrafiltered CFs obtained from 11 patient’s plasma induced a significant increase of the MN frequency peaking at day 15. Thereafter, a slow MN frequency decline was observed and the baseline frequency was reached after 180 days. A significant relationship was found between the MN frequency observed in lymphocytes of patients after therapy and the genotoxic CFs activity present in their plasma (P=0.019). These findings suggest that induces a significant increase in the MN frequency of peripheral blood lymphocytes, as well as the formation of transferable CFs which persist for at least 60 days after administration of the radionuclide. The presence of these CFs might be responsible of chromosome aberrations often observed in cultured lymphocytes following X-ray exposure. The possibility of reducing the genotoxic activity of radionuclide therapy by chemoprevention of CFs with antioxidant drugs remains to be explored.

Formation of micronuclei and of clastogenic factor(s) in patients receiving therapeutic doses of iodine-131

GEMIGNANI, FEDERICA;MARIANI, GIULIANO;ROSSI, ANNA MARIA;MIGLIORE, LUCIA;BARALE, ROBERTO
2002-01-01

Abstract

The micronucleus (MN) assay in peripheral blood lymphocytes was applied to assess the genotoxic potential of a single dose of iodine-131 () given to six patients for ablation of thyroid remnants after total thyroidectomy. Lymphocytes were taken at various times after therapy (from 2 to 180 days), and evaluated for the presence of MN in the binucleated cells identified after blocking cytokynesis with cytochalasin B. The presence of ultrafiltered, low-molecular weight, clastogenic factor(s) (CFs) in the plasma of 11 patient undergoing therapy was also sequentially assessed. A significantly increased MN frequency was observed in lymphocytes of patients as soon as the first sampling time (2 days after therapy), multifactor analysis of variance (MANOVA): P<0.0001, peaking at day 7 at almost four-fold the spontaneous frequency observed in the pre-therapy samples. MN frequency slowly declined thereafter, reaching the baseline levels at the 180-day time point. When tested against peripheral blood lymphocytes from a healthy donor, the ultrafiltered CFs obtained from 11 patient’s plasma induced a significant increase of the MN frequency peaking at day 15. Thereafter, a slow MN frequency decline was observed and the baseline frequency was reached after 180 days. A significant relationship was found between the MN frequency observed in lymphocytes of patients after therapy and the genotoxic CFs activity present in their plasma (P=0.019). These findings suggest that induces a significant increase in the MN frequency of peripheral blood lymphocytes, as well as the formation of transferable CFs which persist for at least 60 days after administration of the radionuclide. The presence of these CFs might be responsible of chromosome aberrations often observed in cultured lymphocytes following X-ray exposure. The possibility of reducing the genotoxic activity of radionuclide therapy by chemoprevention of CFs with antioxidant drugs remains to be explored.
2002
Ballardin, M; Gemignani, Federica; Bodei, L; Mariani, Giuliano; Ferdeghini, M; Rossi, ANNA MARIA; Migliore, Lucia; Barale, Roberto
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S1383571801003230-main.pdf

solo utenti autorizzati

Tipologia: Versione finale editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 72.79 kB
Formato Adobe PDF
72.79 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/178550
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 38
  • ???jsp.display-item.citation.isi??? 34
social impact