Much of the morbidity of intracranial meningiomas is related to the degree of tumour vascularity and the extent of peritumoural vasogenic oedema. Several studies have shown that vascular endothelial growth factor (VEGF) is up-regulated in meningiomas. although its relationship with tumour vasculature is still unclear. In order to better understand the angiogenic assessment of intracranial meningiomas, we analysed its vascular pattern, both as number and as morphologic configuration of microvessels. Moreover, we investigated the mRNA-VEGF expression, relating this expression to vascular pattern. A total of 40 intracranial meningiomas, classified as benign (31 cases), atypical (7 cases), and anaplastic (2 cases) were analysed. RT-PCR analyses of mRNA-VEGF and competitive-PCR were performed. VEGF expression and microvessel density (MVD) were also immunohistochemically investigated. Grade II-III meningiomas showed numerous small microvessels (mean: 34), while the majority of Grade I showed few larger vessels (mean: 13.09) (P=0.000003). A microvessel pattern overlapping into atypical subtype was found in eignt of the 31 (25.8%) Grade I meningiomas. A significant association was found between grading and vascular pattern(P=0.0002), as well as between the MVD and the immunohistochemical expression of VEGF (P=0.0005). The expression of mRNA agreed with the immunohistochemical expression of the protein (P<0.0001). A total of 39 cases expressed the 121 VEGF isoform and, among these, 2 8 cases also expressed the 165 isoform. Only 9 cases expressed both isoforms 165 and 189. Grade II and III meningiomas showed a preponderant expression of soluble isoforms (12 1 and 16 5). These results prompt us to speculate that the microvessel pattern could underlie a higher metabolic demand, probably due to a rapid growth with a consequent worse clinical behaviour of the tumour. In this sense, the vascular pattern may be used as a prognostic factor, in order to mostly focus attention on those Grade I meningiomas which have a higher likelihood of either recurrence or development of perilesional oedema. The pattern of vasculature itself seems to be dependent on the types of VEGF isoforms: the Grade II-III meningiomas (that presented numerous microvessels) expressed the soluble isoforms 121 and 16-5. while the isoform 189 was more frequently detected in Grade I meningiomas.
|Autori interni:||BOLDRINI, LAURA|
PARENTI, GIULIANO FRANCESCO
|Autori:||PISTOLESI S; BOLDRINI L; GISFREDI S; DE IESO K; CAMACCI T; CANIGLIA M; LUPI G; LEOCATA P; BASOLO F; PINGITORE R; PARENTI G; FONTANINI G|
|Titolo:||Angiogenesis in intracranial meningiomas: immunohistochemical and molecular study|
|Anno del prodotto:||2004|
|Digital Object Identifier (DOI):||10.1111/j.1365-2990.2003.0516.x|
|Appare nelle tipologie:||1.1 Articolo in rivista|