This study evaluated the release of Th1 and Th2 cytokines from human lymphomononuclear cells (LMC) in response to purified human (HI) or bovine (BI) islets, and the role of long-term (3-4 weeks) islet culture and removal of monocyte-macrophage cells. The results showed that HI and BI caused a similar increase of the release of gamma interferon (IFN), IL-2 and IL-6 from LMC, whereas BI had a more marked effect than HI on IL-10 release. Culturing the islets had possible positive effects (reduction of IFN and IL-2), but also potentially negative effects (increase of TNF). Removal of monocyte-macrophage cells determined a significant reduction of IL-6, IL-10 and TNF production in response to xeno-islets.
|Autori:||MARSELLI L; MARCHETTI P; TELLINI C; GIANNARELLI R; LENCIONI C; DEL GUERRA S; LUPI R; CARMELLINI M; MOSCA F; NAVALESI R|
|Titolo:||Lymphokine release from human lymphomononuclear cells after co-culture with isolated pancreatic islets: effects of islet species, long-term culture, and monocyte-macrophage cell removal|
|Anno del prodotto:||2000|
|Digital Object Identifier (DOI):||10.1006/cyto.1999.0583|
|Appare nelle tipologie:||1.1 Articolo in rivista|