In this study, we assessed the expression stability of eight genes (GADPH, ACTB, 18S, YWHAZ, SDHA, HMBS, SF3A1, and EEF1A) in the white blood cells of lactating buffalos and their possible use as reference genes for studies on growth hormone (GH)-treated animals. All of the genes showed acceptable stability according to the threshold values suggested by some of the software that was used to analyze them, although the differences between the most stable (SF3A1 and ACTB) and the least stable (18S) were considerable. GH treatment did not influence their expression levels.

Selecting reference genes in the white blood cells of buffalos treated with recombinant growth hormone

CASTIGLIEGO, LORENZO;ARMANI, ANDREA;GIANFALDONI, DANIELA;GUIDI, ALESSANDRA
2010

Abstract

In this study, we assessed the expression stability of eight genes (GADPH, ACTB, 18S, YWHAZ, SDHA, HMBS, SF3A1, and EEF1A) in the white blood cells of lactating buffalos and their possible use as reference genes for studies on growth hormone (GH)-treated animals. All of the genes showed acceptable stability according to the threshold values suggested by some of the software that was used to analyze them, although the differences between the most stable (SF3A1 and ACTB) and the least stable (18S) were considerable. GH treatment did not influence their expression levels.
Castigliego, Lorenzo; Armani, Andrea; Li, X; Grifoni, G; Gianfaldoni, Daniela; Guidi, Alessandra
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/190775
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