4-(Dimethylamino)pyridine-coated gold nanoparticles (DMAP-Au NPs) were synthesized, characterised and their interaction with DNA and living cells was analysed. Concerning the interaction of the DMAP-Au NPs with DNA, absorbance titrations indicate that a non-covalent interaction between DNA and the external surface of the NPs does take place. The binding constant was evaluated to be (2.8 ± 0.8) 9 105 M-1. Exposure of cultured cells to NPs revealed a dose-dependent effect on cell proliferation which was increased or reduced in dependence of DMAP-Au NPs concentrations. Subcellular localisation by transmission electron microscopy showed mitochondrial and nuclear localisations of NPs, thus suggesting their direct involvement in the mitochondrial alterations observed and a possible direct interaction with cell DNA. These findings clearly indicate that DMAP-Au NPs can strongly interact with living cells and confirm the importance of systematic evaluations of NPs properties, also in the perspective of their arising diagnostic and therapeutic applications

Analysis of 4-dimethylaminopyridine (DMAP)-gold nanoparticles behaviour in solution and of their interaction with calf thymus DNA and living cells

BIVER, TARITA;CORTI, ALESSANDRO;LORENZINI, EVELINA;MASINI, MATILDE;PAOLICCHI, ALDO;PUCCI, ANDREA;RUGGERI, GIACOMO;
2012

Abstract

4-(Dimethylamino)pyridine-coated gold nanoparticles (DMAP-Au NPs) were synthesized, characterised and their interaction with DNA and living cells was analysed. Concerning the interaction of the DMAP-Au NPs with DNA, absorbance titrations indicate that a non-covalent interaction between DNA and the external surface of the NPs does take place. The binding constant was evaluated to be (2.8 ± 0.8) 9 105 M-1. Exposure of cultured cells to NPs revealed a dose-dependent effect on cell proliferation which was increased or reduced in dependence of DMAP-Au NPs concentrations. Subcellular localisation by transmission electron microscopy showed mitochondrial and nuclear localisations of NPs, thus suggesting their direct involvement in the mitochondrial alterations observed and a possible direct interaction with cell DNA. These findings clearly indicate that DMAP-Au NPs can strongly interact with living cells and confirm the importance of systematic evaluations of NPs properties, also in the perspective of their arising diagnostic and therapeutic applications
Biver, Tarita; Corti, Alessandro; N., Eltugral; Lorenzini, Evelina; Masini, Matilde; Paolicchi, Aldo; Pucci, Andrea; Ruggeri, Giacomo; F., Secco; M., Venturini
File in questo prodotto:
File Dimensione Formato  
66/00832482309354073552080853257955179396

solo utenti autorizzati

Tipologia: Versione finale editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 737.82 kB
Formato Unknown
737.82 kB Unknown   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/192835
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 10
social impact