A total of 12 wild hares were captured by coursing with 3-4 dogs (greyhounds or lurches) in a protected area of Florence province (X= 1667003 Y= 4844543, ref. Rome, 1940) for the following release in a low-density area. Immediately after capture the eyes of 6 hares were equipped with an obscuring cloth tend (treated) while the other 6 were normally handled to the wooden darkened capture-boxes without blinding their eyes (control). All the hares remained inside the capture-boxes before blood drawing for a variable period of time. For sample collection all the hares were physically restrained with their eyes immediately covered and blood was always collected within 1 - 2 minutes by the auricular vein. Blood samples (plasma) were analyzed for glucose, cholesterol, triglycerides, CK, AST, ALT, NEFA, BUN, total protein, albumins, globulins and cortisol concentrations. Body temperature, heart and respiratory rate, sex, and age were evaluated in each hare. The hares were then equipped with a radio tag (Biotrak TW3) and released again to the wildness. Results showed that the reference values (means ± SE) were as follows: hares equipped with the obscuring cloth tend in the net: temperature 38.8±0.32 °C, respiratory rate 95±9 n/min, hearth rate 122±15 n/min, glucose 104±26 mg/dl, cholesterol 82±15mg/dl, triglycerides 40±6, CK 3701±1629 U/l, AST 141±28 U/l, ALT 42±3U/l, NEFA 50±2mg/l, BUN 49±6mg/dl, total protein 5.9±0.31, albumins 4.9±0.20 d/dl, globulins 1.0±0.20 g/dl, cortisol 22±4 μg/dl; control hares: temperature 37.2±0.37 °C, respiratory rate 81±10 n/min, hearth rate 106±17 n/min, glucose 87±33 mg/dl, Cholesterol 100±19mg/dl, triglycerides 32±7, CK 5510±1995 U/l, AST 192±34 U/l, ALT 31±4U/l, NEFA 50±2mg/l, BUN 58±8 mg/dl, total protein 5.7±0.38, albumins 4.7±0.24 d/dl, globulins 1.0±0.24 g/dl, cortisol 15±5 μg/dl. Temperature and ALT were significantly lower in the control group compared to the treated group but the AST/ALT ratio was significantly higher in the control group compared to the treated group. Only one hare belonging to treated hares died within the 30 days following release. The incidence of subclinical stress was 28,6 % in the treated hares and 80,0 % in the control hares (χ.2c = 1.37, p = 25.3%). The results indicate that the technique to put an obscuring cloth tend on the eyes of hares remained trapped in the nets, reduce the subclinical stress caused during capturing.

Reducing visual stimulations in European hares (Lepus europaeus Pallas) captured for translocation

BAGLIACCA, MARCO;PACI, GISELLA
2008-01-01

Abstract

A total of 12 wild hares were captured by coursing with 3-4 dogs (greyhounds or lurches) in a protected area of Florence province (X= 1667003 Y= 4844543, ref. Rome, 1940) for the following release in a low-density area. Immediately after capture the eyes of 6 hares were equipped with an obscuring cloth tend (treated) while the other 6 were normally handled to the wooden darkened capture-boxes without blinding their eyes (control). All the hares remained inside the capture-boxes before blood drawing for a variable period of time. For sample collection all the hares were physically restrained with their eyes immediately covered and blood was always collected within 1 - 2 minutes by the auricular vein. Blood samples (plasma) were analyzed for glucose, cholesterol, triglycerides, CK, AST, ALT, NEFA, BUN, total protein, albumins, globulins and cortisol concentrations. Body temperature, heart and respiratory rate, sex, and age were evaluated in each hare. The hares were then equipped with a radio tag (Biotrak TW3) and released again to the wildness. Results showed that the reference values (means ± SE) were as follows: hares equipped with the obscuring cloth tend in the net: temperature 38.8±0.32 °C, respiratory rate 95±9 n/min, hearth rate 122±15 n/min, glucose 104±26 mg/dl, cholesterol 82±15mg/dl, triglycerides 40±6, CK 3701±1629 U/l, AST 141±28 U/l, ALT 42±3U/l, NEFA 50±2mg/l, BUN 49±6mg/dl, total protein 5.9±0.31, albumins 4.9±0.20 d/dl, globulins 1.0±0.20 g/dl, cortisol 22±4 μg/dl; control hares: temperature 37.2±0.37 °C, respiratory rate 81±10 n/min, hearth rate 106±17 n/min, glucose 87±33 mg/dl, Cholesterol 100±19mg/dl, triglycerides 32±7, CK 5510±1995 U/l, AST 192±34 U/l, ALT 31±4U/l, NEFA 50±2mg/l, BUN 58±8 mg/dl, total protein 5.7±0.38, albumins 4.7±0.24 d/dl, globulins 1.0±0.24 g/dl, cortisol 15±5 μg/dl. Temperature and ALT were significantly lower in the control group compared to the treated group but the AST/ALT ratio was significantly higher in the control group compared to the treated group. Only one hare belonging to treated hares died within the 30 days following release. The incidence of subclinical stress was 28,6 % in the treated hares and 80,0 % in the control hares (χ.2c = 1.37, p = 25.3%). The results indicate that the technique to put an obscuring cloth tend on the eyes of hares remained trapped in the nets, reduce the subclinical stress caused during capturing.
2008
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/193673
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