he spontaneous gelation of RWDW (R = L-arginine, W = L-tryptophan, D = Laspartic acid) solutions in cell culture medium was investigated. The aqueous solutions were prepared and a total of 100 mM, pH 3.5 phosphate buffer was prepared by dissolving 1.303 g of KH 2PO 4 in 100 mL of 0.042% H 3PO 4 with 50 mM and pH 3.5 phosphate buffer was obtained. Light scattering measurements were performed on oligopeptide solutions obtained by sequential dilution of concentrated solutions in 50 mM, pH 3.5 phosphate buffer. Solutions were irradiated at 320 nm and the scattered light was collected at 90° in the 310-330 nm range. The cytotoxicity of RWDW was evaluated by determining the peptide concentration that brings about 50% reduction of cell viability and proliferation. The results revealed that the oligopeptide is not cytotoxic, at least up to 70 mg/L concentration.
|Autori:||DESII A; CHIELLINI F; STEFANO R.D; TINÉ M.R; SOLARO R|
|Titolo:||Hydrogel Scaffolds by Self–Assembly of a Complementary Ionic Tetrapeptide|
|Anno del prodotto:||2010|
|Digital Object Identifier (DOI):||10.1002/pola.23841|
|Appare nelle tipologie:||1.1 Articolo in rivista|