DNA damage, mainly single strand breaks, was evaluated by single cell gel electrophoresis, in leukocytes of 36 healthy and 14 thyroid cancer-affected children prior to radio-therapy. The children come from the Gomel region, one of the areas most heavily radio-contaminated by the Chernobyl fallout. In addition, leukocytes were treated with a challenge dose of bleomycin BLM, 1.5 mgrml., to assess the presence of an adaptive response AR. potentially resulting from chronic exposure to radionuclides. As controls, 13 children living in Pisa Italy. were enrolled in the study. Children with thyroid cancer show higher p-0.001. DNA damage than healthy ones. No difference was found between healthy children from Gomel and from Pisa. A reduction in the response to BLM was significantly linked to low plasma levels of FT4 hormone . . . 137 p-0.0001 , to the presence of the tumor p-0.002 , to being female p-0.02 , and to a higher Cs body burden p-0.03.. q1998 Elsevier Science B.V. All rights reserved.
Comet assay on children's leukocytes 8 years after the Chernobyl disaster
FRENZILLI, GIADA;BARALE, ROBERTO
1998-01-01
Abstract
DNA damage, mainly single strand breaks, was evaluated by single cell gel electrophoresis, in leukocytes of 36 healthy and 14 thyroid cancer-affected children prior to radio-therapy. The children come from the Gomel region, one of the areas most heavily radio-contaminated by the Chernobyl fallout. In addition, leukocytes were treated with a challenge dose of bleomycin BLM, 1.5 mgrml., to assess the presence of an adaptive response AR. potentially resulting from chronic exposure to radionuclides. As controls, 13 children living in Pisa Italy. were enrolled in the study. Children with thyroid cancer show higher p-0.001. DNA damage than healthy ones. No difference was found between healthy children from Gomel and from Pisa. A reduction in the response to BLM was significantly linked to low plasma levels of FT4 hormone . . . 137 p-0.0001 , to the presence of the tumor p-0.002 , to being female p-0.02 , and to a higher Cs body burden p-0.03.. q1998 Elsevier Science B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
