Captivity rearing of wild pheasant is changing from an experimental to a commercial tecnology. Good results are observed after pheasant release of the offspring obtained from captured animals, therefore we wanted to test the genetic and productive characteristics of wild pheasants living in different protected areas of Central Italy. In order to estimate the level of genetic variability present in the natural populationa of Phasianus colchicus, 99 samples of wild pheasants sampled in 6 differeent locations of Central Italy were genotyped with 3 microsatellite loci (GUJ23, GUJ59 and GUJ86), using pairs of primers developed for Japanese quail that were able to amplify in Phasianus colchicus and resulted polymorphic. In the meantime a sample of wild pheasant captured in different protected areas of the province of Florence were transferred in a breeding farm and the productive performance were recorded. The molecular data obtained were used to calculate the genetic differentiation within and between different pheasant populations (AMOVA, F-statistic)and a PCA was used to visualize the data. The observed homozygosity ranged between 0.33+/- 0.12 and 1.89% of molecular variance resulted located within populations. The mean Fis (inbreeding) resulted 0.234 while the mean Fst (differentiation between population) resulted 0.084. The productive performance of the captured pheasants confirmed the lower performance which can be obtained by just captured wild pheasant in respect to the offspring of the captured pheasants and the rerared populations.
Caratterizzazione, conservazione e miglioramento dei ceppi locali di fagiano (Phasianus colchicus L.). Primi risultati
BAGLIACCA, MARCO;PACI, GISELLA;MANI, PAOLO;
2003-01-01
Abstract
Captivity rearing of wild pheasant is changing from an experimental to a commercial tecnology. Good results are observed after pheasant release of the offspring obtained from captured animals, therefore we wanted to test the genetic and productive characteristics of wild pheasants living in different protected areas of Central Italy. In order to estimate the level of genetic variability present in the natural populationa of Phasianus colchicus, 99 samples of wild pheasants sampled in 6 differeent locations of Central Italy were genotyped with 3 microsatellite loci (GUJ23, GUJ59 and GUJ86), using pairs of primers developed for Japanese quail that were able to amplify in Phasianus colchicus and resulted polymorphic. In the meantime a sample of wild pheasant captured in different protected areas of the province of Florence were transferred in a breeding farm and the productive performance were recorded. The molecular data obtained were used to calculate the genetic differentiation within and between different pheasant populations (AMOVA, F-statistic)and a PCA was used to visualize the data. The observed homozygosity ranged between 0.33+/- 0.12 and 1.89% of molecular variance resulted located within populations. The mean Fis (inbreeding) resulted 0.234 while the mean Fst (differentiation between population) resulted 0.084. The productive performance of the captured pheasants confirmed the lower performance which can be obtained by just captured wild pheasant in respect to the offspring of the captured pheasants and the rerared populations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
