In an effort to identify novel ligands possessing high affinity and selectivity for the A2B AR subtype, we further investigated the class of 3-aryl[1,2,4]triazino[4,3-a]benzimidazol-4(10H)-ones V, previously disclosed by us as selective A1 AR antagonists. Preliminary assays on a number of triazinobenzimidazoles derived from our "in-house" collection revealed that all the derivatives selected showed significant affinity at A 2B AR, no affinity at A3 AR, and various degrees of selectivity toward A1 and A2A ARs. Investigation of a new series featuring modified substituents at the 10-position (4′-chlorophenyl or phenylethyl groups), and a chlorine atom at the 7-position (X) of the triazinobenzimidazole nucleus, yielded highly potent and selective A 2B AR antagonists. The presence of a pendant 3-phenyl ring appears to hamper the interaction with A2A AR, conferring high A 2B/A2A AR selectivity. Derivative 13 (X = Cl, R = C 6H5) is the most potent and selective compound, with an IC50 of 3.10 nM at A2B AR and no affinity at A 1, A2A, and A3 ARs

3-Aryl-[1,2,4]triazino[4,3-a]benzimidazol-4(10H)-one: A Novel Template for the Design of Highly Selective A(2B) Adenosine Receptor Antagonists.

TALIANI, SABRINA
Primo
;
BARRESI, ELISABETTA;SIMORINI, FRANCESCA;SALERNO, SILVIA;LA MOTTA, CONCETTINA;MARINI, ANNA MARIA;DANIELE, SIMONA;TRINCAVELLI, MARIA LETIZIA;MARTINI, CLAUDIA
Penultimo
;
DA SETTIMO PASSETTI, FEDERICO
Ultimo
2012

Abstract

In an effort to identify novel ligands possessing high affinity and selectivity for the A2B AR subtype, we further investigated the class of 3-aryl[1,2,4]triazino[4,3-a]benzimidazol-4(10H)-ones V, previously disclosed by us as selective A1 AR antagonists. Preliminary assays on a number of triazinobenzimidazoles derived from our "in-house" collection revealed that all the derivatives selected showed significant affinity at A 2B AR, no affinity at A3 AR, and various degrees of selectivity toward A1 and A2A ARs. Investigation of a new series featuring modified substituents at the 10-position (4′-chlorophenyl or phenylethyl groups), and a chlorine atom at the 7-position (X) of the triazinobenzimidazole nucleus, yielded highly potent and selective A 2B AR antagonists. The presence of a pendant 3-phenyl ring appears to hamper the interaction with A2A AR, conferring high A 2B/A2A AR selectivity. Derivative 13 (X = Cl, R = C 6H5) is the most potent and selective compound, with an IC50 of 3.10 nM at A2B AR and no affinity at A 1, A2A, and A3 ARs
Taliani, Sabrina; Pugliesi, I; Barresi, Elisabetta; Simorini, Francesca; Salerno, Silvia; LA MOTTA, Concettina; Marini, ANNA MARIA; Cosimelli, B; Cosconati, S; Di Maro, S; Marinelli, L; Daniele, Simona; Trincavelli, MARIA LETIZIA; Greco, G; Novellino, E; Martini, Claudia; DA SETTIMO PASSETTI, Federico
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/199631
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