The regeneration of shoots of Echinacea angustifolia DC. in vitro, using sections of flower stalks of open field E. angustifolia adult plants, was achieved. These sections produced the formation of shoots directly or from morphogenetic callus on a modified MS culture basal medium, named CH, supplemented with 0.5 mg L-1 6-benzilamminopurine (BA). The in vitro proliferation was carried out using the same basal medium after two previous subcultures with 0.25 mg L-1 BA and active charcoal (5 mg L-1) without plant growth regulators. Callus cultures were also obtained from leaf segments cultured on CH basal medium with 3 mg L-1 BA and 0.5 mg L-1 indole-3-butyric acid (IBA). The secondary metabolite content of the different plant material obtained from the in vitro cultures was defined. Caffeic acid derivatives (chlorogenic, caffeic, caftaric, cichoric acids and echinacoside) and alkamide [dodeca-2E,4E, 8Z,10E-tetraenoic acid isobuthylamide] (chosen as representative for the alkamides) were considered as markers for the quali-quantitative LC-DAD-ESI-MS analysis. In vitro shoots in different growth phases showed significant differences in the production of alkamides or caffeolquinic acid derivatives giving the possibility to select the plant material on the basis of its secondary metabolite content.
PHYTOCHEMICAL CHARACTERISATION OF IN VITRO REGENERATED SHOOTS OF ECHINACEA ANGUSTIFOLIA DC.
BERTOLI, ALESSANDRA;LUCCHESINI, MARIELLA;PISTELLI, LAURA;PISTELLI, LUISA
2009
Abstract
The regeneration of shoots of Echinacea angustifolia DC. in vitro, using sections of flower stalks of open field E. angustifolia adult plants, was achieved. These sections produced the formation of shoots directly or from morphogenetic callus on a modified MS culture basal medium, named CH, supplemented with 0.5 mg L-1 6-benzilamminopurine (BA). The in vitro proliferation was carried out using the same basal medium after two previous subcultures with 0.25 mg L-1 BA and active charcoal (5 mg L-1) without plant growth regulators. Callus cultures were also obtained from leaf segments cultured on CH basal medium with 3 mg L-1 BA and 0.5 mg L-1 indole-3-butyric acid (IBA). The secondary metabolite content of the different plant material obtained from the in vitro cultures was defined. Caffeic acid derivatives (chlorogenic, caffeic, caftaric, cichoric acids and echinacoside) and alkamide [dodeca-2E,4E, 8Z,10E-tetraenoic acid isobuthylamide] (chosen as representative for the alkamides) were considered as markers for the quali-quantitative LC-DAD-ESI-MS analysis. In vitro shoots in different growth phases showed significant differences in the production of alkamides or caffeolquinic acid derivatives giving the possibility to select the plant material on the basis of its secondary metabolite content.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
