The water status of maize seedlings stressed by water shortage was characterized by estimating the leaf water potential and the pressure and osmotic potential by a pressure/volume method. The protein pattern of water-stressed and -unstressed maize leaves and the content of free amino acids were examined. A decrease in water potential caused by water stress was not accompanied by a corresponding reduction in turgor pressure, which remained at the same level of the well-watered plants. An active osmotic adjustment apparently occurs in maize grown under water stress. Drought treatment caused an increase in total free amino acid content and a consistent rearrangement of the amino acid pool, with an accumulation of alanine, arginine, valine, aspartate, serine, threonine and tyrosine, and a decrease in glutamic acid, glycine and methionine. The results from native gel electrophoresis and electrophoresis on dodecylsulfate polyacrylamide gels of soluble proteins from leaves of maize plants grown under wet and drought conditions were compare
Change in free amino acid and protein pattern of maize seedling under water stress.
RANIERI, ANNAMARIA;BERNARDI, RODOLFO;
1989-01-01
Abstract
The water status of maize seedlings stressed by water shortage was characterized by estimating the leaf water potential and the pressure and osmotic potential by a pressure/volume method. The protein pattern of water-stressed and -unstressed maize leaves and the content of free amino acids were examined. A decrease in water potential caused by water stress was not accompanied by a corresponding reduction in turgor pressure, which remained at the same level of the well-watered plants. An active osmotic adjustment apparently occurs in maize grown under water stress. Drought treatment caused an increase in total free amino acid content and a consistent rearrangement of the amino acid pool, with an accumulation of alanine, arginine, valine, aspartate, serine, threonine and tyrosine, and a decrease in glutamic acid, glycine and methionine. The results from native gel electrophoresis and electrophoresis on dodecylsulfate polyacrylamide gels of soluble proteins from leaves of maize plants grown under wet and drought conditions were compareI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
