3-Iodothyronamine (T1AM) is regarded as a hormone-like substance thanks to its endogenous nature, itsinteraction with specific receptors trace amine-associated receptor 1 and its biological effects. We characterized T1AM transport and conversion in an in vitro culture of H9c2 murine cells, after a T1AM bolus injection. Samples of cell medium culture and cell lysate were assayed by high-performance liquid chromatography coupled to tandem mass spectrometry. We performed comparative experiments by adding to T1AM bolus amino oxidase inhibitors as iproniazid, pargyline (monoamine oxidase, MAO inhibitors), aminoguanidine, and semicarbazide(semicarbazide-sensitive amino oxidase, SSAO inhibitors). A mathematical model was developed, based on the assumption that T1AM is transported with a mechanism that is typical of hormone transport (i.e., EGF or insulin). We noticed that surface receptors downregulation could play a major role in T1AM dynamics. We also estimated that T1AM catabolism is mainly affected by MAO inhibitors, which produce a dramatic decrease in the kinetic constants related to T1AM degradation, while no significant changes were observed in experiments with SSAO inhibitors.
|Autori:||Orsi G; Ghelardoni S; Saba A; Zucchi R; Vozzi G|
|Titolo:||Characterization of 3-Iodothyronamine In Vitro Dynamics by Mathematical Modeling|
|Anno del prodotto:||2014|
|Digital Object Identifier (DOI):||10.1007/s12013-013-9680-y|
|Appare nelle tipologie:||1.1 Articolo in rivista|