The paper production process is significantly affected by direct and indirect effects of microorganism proliferation. Microorganisms can be introduced in different steps. Some microorganisms find optimum growth conditions and proliferate along the production process, affecting both the end product quality and the production efficiency. The increasing need to reduce water consumption for economic and environmental reasons has led most paper mills to reuse water through increasingly closed cycles, thus exacerbating the bacterial proliferation problem. In this work, microbial communities in a paper mill located in Italy were characterized using both culture-dependent and independent methods. Fingerprinting molecular analysis and 16S rRNA library construction coupled with bacterial isolation were performed. Results highlighted that the bacterial community composition was spatially homogeneous along the whole process, while it was slightly variable over time. The culture-independent approach confirmed the presence of the main bacterial phyla detected with plate counting, coherently with earlier cultivation studies (Proteobacteria, Bacteroidetes, and Firmicutes), but with a higher genus diversification than previously observed. Some minor bacterial groups, not detectable by cultivation, were also detected in the aqueous phase. Overall, the population dynamics observed with the double approach led us to hypothesize a possible role of suspended bacteria in the re-formation mechanisms of resistant biofilms.

Bacterial Community Characterization in Paper Mill White Water

CHIELLINI, CAROLINA
Primo
;
IANNELLI, RENATO
Secondo
;
PETRONI, GIULIO
Ultimo
2014

Abstract

The paper production process is significantly affected by direct and indirect effects of microorganism proliferation. Microorganisms can be introduced in different steps. Some microorganisms find optimum growth conditions and proliferate along the production process, affecting both the end product quality and the production efficiency. The increasing need to reduce water consumption for economic and environmental reasons has led most paper mills to reuse water through increasingly closed cycles, thus exacerbating the bacterial proliferation problem. In this work, microbial communities in a paper mill located in Italy were characterized using both culture-dependent and independent methods. Fingerprinting molecular analysis and 16S rRNA library construction coupled with bacterial isolation were performed. Results highlighted that the bacterial community composition was spatially homogeneous along the whole process, while it was slightly variable over time. The culture-independent approach confirmed the presence of the main bacterial phyla detected with plate counting, coherently with earlier cultivation studies (Proteobacteria, Bacteroidetes, and Firmicutes), but with a higher genus diversification than previously observed. Some minor bacterial groups, not detectable by cultivation, were also detected in the aqueous phase. Overall, the population dynamics observed with the double approach led us to hypothesize a possible role of suspended bacteria in the re-formation mechanisms of resistant biofilms.
Chiellini, Carolina; Iannelli, Renato; Raissa, Lena; Maria, Gullo; Petroni, Giulio
File in questo prodotto:
File Dimensione Formato  
Acceptation letter.pdf

non disponibili

Tipologia: Altro materiale allegato
Licenza: Importato da Ugov Ricerca - Accesso privato/ristretto
Dimensione 136.29 kB
Formato Adobe PDF
136.29 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
2014 Chiellini etal.Bioresources 9(2) 2541-2559. Bacterial Community Characterization in Paper Mill White Water.pdf

accesso aperto

Tipologia: Documento in Post-print
Licenza: Importato da Ugov Ricerca - Accesso privato/ristretto
Dimensione 663.63 kB
Formato Adobe PDF
663.63 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/369467
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 8
social impact