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EnglishThe intragastric host-mediated assay (h.m.a.) was devised and carried out with a view to assessing the formation of direct mutagens in the gastrointestinal tract of mammals. The h.m.a. consists in the injection of nitrosable compounds. NaNO 2 and cells of the yeast S. pombe, by gavage into the animals' stomachs and in the recovery of the target cells from the faeces for mutation-induction analysis. Methylurea was chosen as a model nitrosable compound, and the effects of nitrosation modulators such as ascorbic acid and thiocyanate were studied. Cimetidine, a drug nitrosable in vitro, was tested with the system. Positive results were obtained only at very large doses and in artificially produced low pH. The new host-mediated assay seems to be efficient in revealing the formation, in vivo, of direct, short-living mutagens.
| Autori interni: | ||
| Autori: | Barale R; Zucconi D; Romano M; Loprieno N | |
| Titolo: | The intragastric host-mediated assay for the assessment of the formation of direct mutagens in vivo. | |
| Anno del prodotto: | 1983 | |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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