AN IMMUNOHISTOCHEMICAL STUDY OF THE PTEN/AKT PATHWAY INVOLVEMENT IN CANINE AND FELINE MAMMARY TUMOURS Asproni P.[1], Millanta F.[1], Ressel L.[2], Vannozzi I.[1], Poli A.P.*[1] [1]Università ~ Pisa, [2]Università ~ Liverpool The PTEN/AKT pathway is strongly involved in cell metabolism, proliferation and survival. Its dysregulation is implicated in several human cancers. The aim of this study was to investigate the role of this pathway in canine (CMTs) and feline mammary tumors (FMTs) by immunohistochemistry (IHC). Fifty CMTs (10 adenomas and 40 carcinomas) and 30 FMTs (30 carcinomas) were submitted to IHC to evaluate PTEN, phospho-AKT and Rictor expression. All the canine adenomas (100%), 25 of 40 (63%) canine carcinomas, and 7 of 30 (23%) FMTs were PTENpositive. In dogs, no adenomas and 15 of 25 (37%) carcinomas expressed phospho-AKT, while 24 of 30 FMTs (82%) were phospho-AKT-positive. One of 10 (10%) canine adenomas (100%), 24 of 40 (60%) canine carcinomas, and 20 of 30 (67%) FMTs were Rictor-positve. In dogs, PTEN correlated with complex carcinomas, lower mitotic index, absence of lymphatic invasion and longer survival; phospho-AKT with simple carcinomas, lymphatic invasion and poorer survival and Rictor with lymphatic invasion. In cats, PTEN correlated with tubulopapillary carcinomas, lower mitotic index, absence of lymphatic invasion and better survival, while phospho-AKT and Rictor with poorer survival. In CMTs, phospho-AKT inversely correlated with PTEN expression and positively with Rictor. In FMTs, PTEN inversely correlated with phospho-AKT and Rictor while phospho-AKT positively correlated with Rictor expression. Our data show a strong PTEN/AKT pathway involvement in behavior worsening of CMT and FMTs. This data could provide a rationale for further studies of this system in veterinary oncology to due prognostic and therapeutic implications. Jiang BH, Liu LZ. PI3K/PTEN signaling in angiogenesis and tumorigenesis. Adv Cancer Res. 2009;102:19-65. Maniscalco L, Iussich S, de Las Mulas JM, et al. Activation of AKT in feline mammary carcinoma: a new prognostic factor for feline mammary tumours. Vet J. 2012;191(1):65-71. Ressel L, Millanta F, Caleri E, Innocenti VM, Poli A. Reduced PTEN protein expression and its prognostic implications in canine and feline mammary tumors. Vet Pathol. 2009;46(5):860-868. Patologia veterinaria/Oncologia comparata Dog and cat, Mammary tumours, PTEN/AKT pathway

AN IMMUNOHISTOCHEMICAL STUDY OF THE PTEN/AKT PATHWAY INVOLVEMENT IN CANINE AND FELINE MAMMARY TUMOURS

MILLANTA, FRANCESCA;VANNOZZI, IACOPO;POLI, ALESSANDRO
2014

Abstract

AN IMMUNOHISTOCHEMICAL STUDY OF THE PTEN/AKT PATHWAY INVOLVEMENT IN CANINE AND FELINE MAMMARY TUMOURS Asproni P.[1], Millanta F.[1], Ressel L.[2], Vannozzi I.[1], Poli A.P.*[1] [1]Università ~ Pisa, [2]Università ~ Liverpool The PTEN/AKT pathway is strongly involved in cell metabolism, proliferation and survival. Its dysregulation is implicated in several human cancers. The aim of this study was to investigate the role of this pathway in canine (CMTs) and feline mammary tumors (FMTs) by immunohistochemistry (IHC). Fifty CMTs (10 adenomas and 40 carcinomas) and 30 FMTs (30 carcinomas) were submitted to IHC to evaluate PTEN, phospho-AKT and Rictor expression. All the canine adenomas (100%), 25 of 40 (63%) canine carcinomas, and 7 of 30 (23%) FMTs were PTENpositive. In dogs, no adenomas and 15 of 25 (37%) carcinomas expressed phospho-AKT, while 24 of 30 FMTs (82%) were phospho-AKT-positive. One of 10 (10%) canine adenomas (100%), 24 of 40 (60%) canine carcinomas, and 20 of 30 (67%) FMTs were Rictor-positve. In dogs, PTEN correlated with complex carcinomas, lower mitotic index, absence of lymphatic invasion and longer survival; phospho-AKT with simple carcinomas, lymphatic invasion and poorer survival and Rictor with lymphatic invasion. In cats, PTEN correlated with tubulopapillary carcinomas, lower mitotic index, absence of lymphatic invasion and better survival, while phospho-AKT and Rictor with poorer survival. In CMTs, phospho-AKT inversely correlated with PTEN expression and positively with Rictor. In FMTs, PTEN inversely correlated with phospho-AKT and Rictor while phospho-AKT positively correlated with Rictor expression. Our data show a strong PTEN/AKT pathway involvement in behavior worsening of CMT and FMTs. This data could provide a rationale for further studies of this system in veterinary oncology to due prognostic and therapeutic implications. Jiang BH, Liu LZ. PI3K/PTEN signaling in angiogenesis and tumorigenesis. Adv Cancer Res. 2009;102:19-65. Maniscalco L, Iussich S, de Las Mulas JM, et al. Activation of AKT in feline mammary carcinoma: a new prognostic factor for feline mammary tumours. Vet J. 2012;191(1):65-71. Ressel L, Millanta F, Caleri E, Innocenti VM, Poli A. Reduced PTEN protein expression and its prognostic implications in canine and feline mammary tumors. Vet Pathol. 2009;46(5):860-868. Patologia veterinaria/Oncologia comparata Dog and cat, Mammary tumours, PTEN/AKT pathway
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/466070
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact