To evaluate the cytologic features that may help in achieving a diagnosis of malignant mammary tumours Cytological specimens and their histopathological samples from subcentimetric mammary nodules in bitches of variable age and breed reached at the Veterinary Teaching Hospital of the University of Pisa from January 2012 to May 2013 were collected. All cytologic samples were evaluated for nineteen specific cytological features as well as for mean nuclear area by NIS-Elements D 3.1 (Nikon). Data were analyzed with Mann-Whitney U test by SPSS 21.0.0 program 40 nodules were included; 25 malignant carcinoma and 15 benign lesions. Reticular or granular chromatin pattern (p=0,026), abundant macrophage infiltration (p=0,01), severe anisokaryosis (p=0,04) and greater mean nuclear area (p=0,019) were frequently observed in samples from malignant tumours and their frequencies statistically higher than in benign tumours Mammary gland tumors are common in dogs and represent the most common neoplasms in sexually intact female dogs(1). To date, unlike in human medicine, cytological diagnosis of canine mammary nodules remains a challenge (2,3). Maybe the systematic observation of chromatin pattern, macrophage infiltration, grade of anisokaryosis and mean nuclear area can help to discriminate between benign and malignant lesion, improving the diagnostic power of canine mammary cytology.
File in questo prodotto:
Non ci sono file associati a questo prodotto.