Friedreich's ataxia (FRDA) is a neurodegenerative disorder associated with cardiomyopathy and diabetes. Effective therapies for FRDA are an urgent unmet need; there are currently no options to prevent or treat this orphan disease. FRDA is caused by reduced expression of the mitochondrial protein frataxin. We have previously demonstrated that pancreatic β-cell dysfunction and death cause diabetes in FRDA. This is secondary to mitochondrial dysfunction and apoptosis but the underlying molecular mechanisms are not known. Here we show that β-cell demise in frataxin deficiency is the consequence of oxidative stress-mediated activation of the intrinsic pathway of apoptosis. The pro-apoptotic Bcl-2 family members Bad, DP5 and Bim are the key mediators of frataxin deficiency-induced β-cell death. Importantly, the intrinsic pathway of apoptosis is also activated in FRDA patients' induced pluripotent stem cell-derived neurons. Interestingly, cAMP induction normalizes mitochondrial oxidative status and fully prevents activation of the intrinsic pathway of apoptosis in frataxin-deficient β-cells and neurons. This preclinical study suggests that incretin analogs hold potential to prevent/delay both diabetes and neurodegeneration in FRDA.
|Autori:||Igoillo-Esteve M;Gurgul-Convey E;Hu A;Romagueira Bichara Dos Santos L;Abdulkarim B;Chintawar S;Marselli L;Marchetti P;Jonas JC;Eizirik DL;Pandolfo M;Cnop M|
|Titolo:||Unveiling a common mechanism of apoptosis in β-cells and neurons in Friedreich's ataxia.|
|Anno del prodotto:||2014|
|Digital Object Identifier (DOI):||10.1093/hmg/ddu745|
|Appare nelle tipologie:||1.1 Articolo in rivista|