The number of seafood species sold onWestern markets is constantly growing and many unconventional species are sold in ethnic food outlets. In this work, 68 ethnic seafood products variously processed were collected from the Italian market and a molecular analysis was performed by sequencing a full cytochrome c oxidase (COI) DNA barcode (FDB, ~655 bp) or a mini COI DNA barcode (MDB, ~139 bp) using universal primers. Barcodes were then compared with sequences available in BOLD and GenBank. In addition, the label information was assessed according to the European legislation. By using the IDs analysis on BOLD a maximum species identity 98% was retrieved for 84% of the sequences. Of these, 67% were unambiguously identified at species level (51.3% of the FDB and 74% of the MDB). Using NCBI BLAST, 74% of the sequences scored a maximum species identity 98%, of which 73% were identified at species level (52% of the FDB and 61% of the MDB). Both databases performed better in mollusk identification. Overall, 45 products (66%) were not correctly labeled according to the European requirements. Finally, the comparison between the molecular and the label analysis highlighted that 48.5% of the products presented discrepancies between labeling and molecular identification. In particular, health implications were highlighted for 2 samples labeled as squid but identified as Lagocephalus spp., a poisonous puffer fish species banned from the EU market. The present results confirm DNA barcoding as a reliable tool for protecting consumers' health and economic interests.

DNA barcoding reveals commercial and health issues in ethnic seafood sold on the Italian market

ARMANI, ANDREA;GUARDONE, LISA;GIANFALDONI, DANIELA;GUIDI, ALESSANDRA;CASTIGLIEGO, LORENZO
2015

Abstract

The number of seafood species sold onWestern markets is constantly growing and many unconventional species are sold in ethnic food outlets. In this work, 68 ethnic seafood products variously processed were collected from the Italian market and a molecular analysis was performed by sequencing a full cytochrome c oxidase (COI) DNA barcode (FDB, ~655 bp) or a mini COI DNA barcode (MDB, ~139 bp) using universal primers. Barcodes were then compared with sequences available in BOLD and GenBank. In addition, the label information was assessed according to the European legislation. By using the IDs analysis on BOLD a maximum species identity 98% was retrieved for 84% of the sequences. Of these, 67% were unambiguously identified at species level (51.3% of the FDB and 74% of the MDB). Using NCBI BLAST, 74% of the sequences scored a maximum species identity 98%, of which 73% were identified at species level (52% of the FDB and 61% of the MDB). Both databases performed better in mollusk identification. Overall, 45 products (66%) were not correctly labeled according to the European requirements. Finally, the comparison between the molecular and the label analysis highlighted that 48.5% of the products presented discrepancies between labeling and molecular identification. In particular, health implications were highlighted for 2 samples labeled as squid but identified as Lagocephalus spp., a poisonous puffer fish species banned from the EU market. The present results confirm DNA barcoding as a reliable tool for protecting consumers' health and economic interests.
Armani, Andrea; Guardone, Lisa; Riccardo La, Castellana; Gianfaldoni, Daniela; Guidi, Alessandra; Castigliego, Lorenzo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/741070
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