From September 2009 to February 2011, 122 coypus (Myocastor coypus) and 74 rats (Rattus norvegicus) were captured employing cage traps in a protected wetland in Tuscany (central Italy). Blood serum samples were collected from the animals and successively examined by the microagglutination test for several serovars of Leptospira: Bratislava, Ballum, Bataviae, Grippothyphosa, Icterohaemorrhagiae, Copenhageni, Mini, Pomona, Zanoni, Sejroe, Hardjo and Tarassovi. Kidney samples were collected from each animal and tested by bacteriological methods and submitted to Polymerase Chain Reaction. Thirty-four (27.87 %) coypu sera were positive to Leptospira interrogans serovar Bratislava, with antibody titers ranging from 1:100 to 1:400; no strain was isolated from coypu by bacteriological examination, while 12 (9.83 %) subjects were positive to PCR. All rats resulted seronegative; thirty-seven (50 %) Leptospira strains were isolated from rat kidneys; 30 were classifi ed as Leptospira interrogans serovar Icterohaemorrhagiae and 7 as Leptospira interrogans serovar Ballum by the cross-agglutination test. Forty-fi ve (60.81 %) rats resulted positive to PCR: 37 subjects positive to bacteriological examination and there were eight from which no strain were isolated from kidneys. These results would seem to suggest the minor zooepidemiological role of coypu in leptospirosis, which is widespread according to literature.

The presence of Leptospira in coypus (Myocastor coypus) and rats (Rattus norvegicus) living in a protected wetland in Tuscany (Italy)

FRATINI, FILIPPO
Primo
;
TURCHI, BARBARA
Secondo
;
EBANI, VALENTINA VIRGINIA;BERTELLONI, FABRIZIO;CERRI, DOMENICO
Ultimo
2015

Abstract

From September 2009 to February 2011, 122 coypus (Myocastor coypus) and 74 rats (Rattus norvegicus) were captured employing cage traps in a protected wetland in Tuscany (central Italy). Blood serum samples were collected from the animals and successively examined by the microagglutination test for several serovars of Leptospira: Bratislava, Ballum, Bataviae, Grippothyphosa, Icterohaemorrhagiae, Copenhageni, Mini, Pomona, Zanoni, Sejroe, Hardjo and Tarassovi. Kidney samples were collected from each animal and tested by bacteriological methods and submitted to Polymerase Chain Reaction. Thirty-four (27.87 %) coypu sera were positive to Leptospira interrogans serovar Bratislava, with antibody titers ranging from 1:100 to 1:400; no strain was isolated from coypu by bacteriological examination, while 12 (9.83 %) subjects were positive to PCR. All rats resulted seronegative; thirty-seven (50 %) Leptospira strains were isolated from rat kidneys; 30 were classifi ed as Leptospira interrogans serovar Icterohaemorrhagiae and 7 as Leptospira interrogans serovar Ballum by the cross-agglutination test. Forty-fi ve (60.81 %) rats resulted positive to PCR: 37 subjects positive to bacteriological examination and there were eight from which no strain were isolated from kidneys. These results would seem to suggest the minor zooepidemiological role of coypu in leptospirosis, which is widespread according to literature.
Fratini, Filippo; Turchi, Barbara; Ebani, VALENTINA VIRGINIA; Bertelloni, Fabrizio; Galiero, Alessia; Cerri, Domenico
File in questo prodotto:
File Dimensione Formato  
2015 Fratini et al., lepto nutrie.pdf

accesso aperto

Tipologia: Versione finale editoriale
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 136.41 kB
Formato Adobe PDF
136.41 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/751078
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 14
social impact