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EnglishFatty acid composition (FAC) of backfat tissue in pigs is an important trait in pig industry mainly for dry-cured ham produc- tion. In pig adipose tissue, variations in FAC contributes to qual- itative characteristics of fresh meat and seasoned products. FAC is influenced by diet, genetics, breed, sex and environmental fac- tors. Several genes affecting fatty acid composition were high- lighted but the regulation of fatty acid (FA) metabolism and fat deposition is complex and not plenty known yet. For the current study we considered eight known SNPs of 5 candidate genes Acetyl-Coenzyme A Carboxylase Alpha (ACACA), Fatty Acid Synthase (FASN), ATP citrate lyase (ACLY), fatty acid elongase 6 (ELOVL6) and stearoyl-CoA desaturase (SCD) chosen for their functions in lipid metabolism and for the genome positions to perform an association study with FA composition in Italian Large White (ILW) breed. A sample of 536 ILW pigs was geno- typed using PCR-RFLP or High Resolution Melting (HRM) PCR. FAC was determined by gas chromatography after after direct trans-esterification of subcutaneous lipids. The results allowed to determine that 37.58% of the total FA of backfat was represent- ed by saturated FA (SFA), 43.70% by monounsaturated FA (MUFA) and 18.29% by polyunsaturated FA (PUFA). The content of individual SFA, MUFA and PUFA of the analyzed tissue was measured. The association between FA and the genotypes of the 5 candidate genes was assessed using a MIXED model and SAS software. Moreover, the coefficient of correlation between the analysed FA was calculated. Genotyping results revealed that the polymorphisms ACACA NM_198837:c.4899G>A, ACACA NM_198837:c.5196T>C, and ELOVL6 XM_003357048:c.533C>T were monomorphic in the analyzed samples. Significant associ- ation with FAC were obtained for the SNP FASN AY183428:g.265T>C with C14 (P=0.005), C18 (P=0.008), C20 (P=0.0006). The SNP SCD AY487830:g.2228T>C was associated to C18:1 (P=0.03). and MUFA (P=0.05). Moreover ACLY NM_001105302:c.2956T>C presented a significant effect on C20 (P=0.05) and C18:2 cis9,trans11(P=0.05). These results high- lighted that the key genes for lipid metabolism considered in the study can regulate backfat FAC of ILW and the analysed polymor- phisms could be markers useful to modulate the subcutaneous FA profile in pigs.
| Titolo: | Association study of FASN, ACLY, ACACA, SCD, ELOVL6 genes with backfat fatty acid profile and intramuscular fat in Italian Large White pigs |
| Autori interni: | |
| Anno del prodotto: | 2015 |
| Abstract: | Fatty acid composition (FAC) of backfat tissue in pigs is an important trait in pig industry mainly for dry-cured ham produc- tion. In pig adipose tissue, variations in FAC contributes to qual- itative characteristics of fresh meat and seasoned products. FAC is influenced by diet, genetics, breed, sex and environmental fac- tors. Several genes affecting fatty acid composition were high- lighted but the regulation of fatty acid (FA) metabolism and fat deposition is complex and not plenty known yet. For the current study we considered eight known SNPs of 5 candidate genes Acetyl-Coenzyme A Carboxylase Alpha (ACACA), Fatty Acid Synthase (FASN), ATP citrate lyase (ACLY), fatty acid elongase 6 (ELOVL6) and stearoyl-CoA desaturase (SCD) chosen for their functions in lipid metabolism and for the genome positions to perform an association study with FA composition in Italian Large White (ILW) breed. A sample of 536 ILW pigs was geno- typed using PCR-RFLP or High Resolution Melting (HRM) PCR. FAC was determined by gas chromatography after after direct trans-esterification of subcutaneous lipids. The results allowed to determine that 37.58% of the total FA of backfat was represent- ed by saturated FA (SFA), 43.70% by monounsaturated FA (MUFA) and 18.29% by polyunsaturated FA (PUFA). The content of individual SFA, MUFA and PUFA of the analyzed tissue was measured. The association between FA and the genotypes of the 5 candidate genes was assessed using a MIXED model and SAS software. Moreover, the coefficient of correlation between the analysed FA was calculated. Genotyping results revealed that the polymorphisms ACACA NM_198837:c.4899G>A, ACACA NM_198837:c.5196T>C, and ELOVL6 XM_003357048:c.533C>T were monomorphic in the analyzed samples. Significant associ- ation with FAC were obtained for the SNP FASN AY183428:g.265T>C with C14 (P=0.005), C18 (P=0.008), C20 (P=0.0006). The SNP SCD AY487830:g.2228T>C was associated to C18:1 (P=0.03). and MUFA (P=0.05). Moreover ACLY NM_001105302:c.2956T>C presented a significant effect on C20 (P=0.05) and C18:2 cis9,trans11(P=0.05). These results high- lighted that the key genes for lipid metabolism considered in the study can regulate backfat FAC of ILW and the analysed polymor- phisms could be markers useful to modulate the subcutaneous FA profile in pigs. |
| Handle: | http://hdl.handle.net/11568/753435 |
| Appare nelle tipologie: | 4.2 Abstract in Atti di convegno |
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