Fatty acid composition (FAC) of backfat tissue in pigs is an important trait in pig industry mainly for dry-cured ham produc- tion. In pig adipose tissue, variations in FAC contributes to qual- itative characteristics of fresh meat and seasoned products. FAC is influenced by diet, genetics, breed, sex and environmental fac- tors. Several genes affecting fatty acid composition were high- lighted but the regulation of fatty acid (FA) metabolism and fat deposition is complex and not plenty known yet. For the current study we considered eight known SNPs of 5 candidate genes Acetyl-Coenzyme A Carboxylase Alpha (ACACA), Fatty Acid Synthase (FASN), ATP citrate lyase (ACLY), fatty acid elongase 6 (ELOVL6) and stearoyl-CoA desaturase (SCD) chosen for their functions in lipid metabolism and for the genome positions to perform an association study with FA composition in Italian Large White (ILW) breed. A sample of 536 ILW pigs was geno- typed using PCR-RFLP or High Resolution Melting (HRM) PCR. FAC was determined by gas chromatography after after direct trans-esterification of subcutaneous lipids. The results allowed to determine that 37.58% of the total FA of backfat was represent- ed by saturated FA (SFA), 43.70% by monounsaturated FA (MUFA) and 18.29% by polyunsaturated FA (PUFA). The content of individual SFA, MUFA and PUFA of the analyzed tissue was measured. The association between FA and the genotypes of the 5 candidate genes was assessed using a MIXED model and SAS software. Moreover, the coefficient of correlation between the analysed FA was calculated. Genotyping results revealed that the polymorphisms ACACA NM_198837:c.4899G>A, ACACA NM_198837:c.5196T>C, and ELOVL6 XM_003357048:c.533C>T were monomorphic in the analyzed samples. Significant associ- ation with FAC were obtained for the SNP FASN AY183428:g.265T>C with C14 (P=0.005), C18 (P=0.008), C20 (P=0.0006). The SNP SCD AY487830:g.2228T>C was associated to C18:1 (P=0.03). and MUFA (P=0.05). Moreover ACLY NM_001105302:c.2956T>C presented a significant effect on C20 (P=0.05) and C18:2 cis9,trans11(P=0.05). These results high- lighted that the key genes for lipid metabolism considered in the study can regulate backfat FAC of ILW and the analysed polymor- phisms could be markers useful to modulate the subcutaneous FA profile in pigs.
Association study of FASN, ACLY, ACACA, SCD, ELOVL6 genes with backfat fatty acid profile and intramuscular fat in Italian Large White pigs
SERRA, ANDREA;
2015-01-01
Abstract
Fatty acid composition (FAC) of backfat tissue in pigs is an important trait in pig industry mainly for dry-cured ham produc- tion. In pig adipose tissue, variations in FAC contributes to qual- itative characteristics of fresh meat and seasoned products. FAC is influenced by diet, genetics, breed, sex and environmental fac- tors. Several genes affecting fatty acid composition were high- lighted but the regulation of fatty acid (FA) metabolism and fat deposition is complex and not plenty known yet. For the current study we considered eight known SNPs of 5 candidate genes Acetyl-Coenzyme A Carboxylase Alpha (ACACA), Fatty Acid Synthase (FASN), ATP citrate lyase (ACLY), fatty acid elongase 6 (ELOVL6) and stearoyl-CoA desaturase (SCD) chosen for their functions in lipid metabolism and for the genome positions to perform an association study with FA composition in Italian Large White (ILW) breed. A sample of 536 ILW pigs was geno- typed using PCR-RFLP or High Resolution Melting (HRM) PCR. FAC was determined by gas chromatography after after direct trans-esterification of subcutaneous lipids. The results allowed to determine that 37.58% of the total FA of backfat was represent- ed by saturated FA (SFA), 43.70% by monounsaturated FA (MUFA) and 18.29% by polyunsaturated FA (PUFA). The content of individual SFA, MUFA and PUFA of the analyzed tissue was measured. The association between FA and the genotypes of the 5 candidate genes was assessed using a MIXED model and SAS software. Moreover, the coefficient of correlation between the analysed FA was calculated. Genotyping results revealed that the polymorphisms ACACA NM_198837:c.4899G>A, ACACA NM_198837:c.5196T>C, and ELOVL6 XM_003357048:c.533C>T were monomorphic in the analyzed samples. Significant associ- ation with FAC were obtained for the SNP FASN AY183428:g.265T>C with C14 (P=0.005), C18 (P=0.008), C20 (P=0.0006). The SNP SCD AY487830:g.2228T>C was associated to C18:1 (P=0.03). and MUFA (P=0.05). Moreover ACLY NM_001105302:c.2956T>C presented a significant effect on C20 (P=0.05) and C18:2 cis9,trans11(P=0.05). These results high- lighted that the key genes for lipid metabolism considered in the study can regulate backfat FAC of ILW and the analysed polymor- phisms could be markers useful to modulate the subcutaneous FA profile in pigs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
