Do nanomosquitocides impact predation of Mesocyclops edax copepods against Anopheles stephensi larvae?

In this study, we reported a cheap method to synthesize silver nanoparticles (AgNP) using the rhyzome extract of Acorus calamus , a nontoxic and eco-friendly material, that worked as reducing and stabilizing agent during the biosynthesis. AgNP were characterized by UV–vis spectrophotometry, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). We investigated the ovicidal, larvicidal, pupicidal and adulticidal properties of A. calamus aqueous rhyzome extract and green-synthesized AgNP against the malaria vector Anopheles stephensi . Furthermore, we evaluated the predation effi ciency of the cyclopoid copepod Mesocyclops edax against larvae of A. stephensi , both in standard laboratory conditions and in an aquatic environment treated with AgNP.